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Literature summary for 3.4.22.60 extracted from

  • Denault, J.B.; Salvesen, G.S.
    Human caspase-7 activity and regulation by its N-terminal peptide (2003), J. Biol. Chem., 278, 34042-34050.
    View publication on PubMed
Search for more literature for 3.4.22.60

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0605
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
0.0646
-
 acetyl-DEVD-4-nitroanilide pH 7.2, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information human caspase-7 is not a nuclear caspase removal of the N-peptide does not allow an active transport or accumulation of human caspase-7 in the nuclei Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases ?
-
 ?
additional information Homo sapiens the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis ?
-
 ?
poly(ADP-ribose) polymerase + H2O Homo sapiens whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 GenBank Acc. No NM 001227
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the N-peptide of caspase-7 must be removed, probably be caspase-3, before efficient activation of the zymogen can occur in vivo. The N-peptide serves to physically sequester the caspase-7 zymogen in a cytosolic location that prevents access by upstream activators, caspase-8, caspase-9 and caspase-10 Homo sapiens
proteolytic modification the 12000 Da and the 11000 Da polypeptides are generated by processing of the CMH-1 protein at Asp198-Ser199 and to a lesser extent at Asp206-Ala207 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-DEVD-4-nitroanilide + H2O
-
 Homo sapiens acetyl-DEVD + 4-nitroaniline
-
 ?
acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
-
 Homo sapiens acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
-
 ?
additional information caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases Homo sapiens ?
-
 ?
additional information the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis Homo sapiens ?
-
 ?
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nucear entry Homo sapiens ?
-
 ?
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.9
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
10.3
-
 acetyl-DEVD-p-nitroanilide pH 7.2, 37°C Homo sapiens