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Literature summary for 3.4.22.60 extracted from

  • Fernandes-Alnemri, T.; Takahashi, A.; Armstrong, R.C.; Krebs, J.; Fritz, L.C.; Tomaselli, K.J.; Wang, L.; Yu, Z.; Croce, C.M.; Salveson, G.; Earnshaw, W.C.; Litwack, G.; Alnemri, E.S.
    Mch3, a novel human apoptotic cysteine protease highly related to CPP32 (1995), Cancer Res., 55, 6045-6052.
    View publication on PubMed
Search for more literature for 3.4.22.60

Cloned(Commentary)

Cloned (Comment) Organism
bacterially expressed Mch3 has intrinsic autocatalytic and autoactivation activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cowpox seroin CrmA very weak inhibitor Homo sapiens
DEVD-aldehyde potent inhibitor Homo sapiens
YVAD-aldehyde weak inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(ADP-ribose) polymerase + H2O Homo sapiens the cleavage of poly(ADP-ribose) polymerase observed during apoptosis cannot solely be attributed to CPP32 but can also be an activity of Mch2alpha ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55210
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification Mch3alpha is made of two subunits derived from a precursor ProMch3alpha. Asp23 and Asp198 are the most likely processing sites. Bacterially expressed Mch3 has intrinsic autocatalytic and autoactivation activity Homo sapiens
proteolytic modification CPP32 can efficiently cleave proMch3alpha Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
T-cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DEVD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens DEVD + 7-amino-4-methylcoumarin
-
 ?
additional information no cleavage of YVAD-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
poly(ADP-ribose) polymerase + H2O
-
 Homo sapiens ?
-
 ?
poly(ADP-ribose) polymerase + H2O the cleavage of poly(ADP-ribose) polymerase observed during apoptosis cannot solely be attributed to CPP32 but can also be an activity of Mch2alpha Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More it is proposed that the 22000 Da peptide and the 12000 Da peptide are two subunits of the enzyme Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000018
-
 DEVD-aldehyde pH 7.5, 37°C Homo sapiens