Literature summary for 3.4.22.60 extracted from

Garcia-Calvo, M.; Peterson, E.P.; Rasper, D.M.; Vaillancourt, J.P.; Zamboni, R.; Nicholson, D.W.; Thornberry, N.A.
Purification and catalytic properties of human caspase family members (1999), Cell Death Differ., 6, 362-369.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	acetyl-DEVD-7-amido-4-methylcoumarin	pH 7.0	Homo sapiens
0.1	-	acetyl-DEVD-7-amido-4-methylcoumarin	pH 7.5	Homo sapiens
0.13	-	acetyl-VQVDGW-amide	pH 7.5, 30°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-DEVD-7-amido-4-methylcoumarin + H2O	iDEVD s the optimal tetrapeptide recognition motif	Homo sapiens	acetyl-DEVD + 7-amino-4-methylcoumarin	-	?
acetyl-VQVDGW-amide + H2O	preferred peptide substrate	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.5	-	acetyl-DEVD-7-amido-4-methylcoumarin	pH 7.0	Homo sapiens
6.3	-	acetyl-DEVD-7-amido-4-methylcoumarin	pH 7.5	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	reaction with acetyl-DEVD-7-amido-4-methylcoumarin	Homo sapiens

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Release 2023.1 (January 2023)