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Literature summary for 3.4.22.56 extracted from

  • Wang, X.H.; Zhang, L.; Mitch, W.E.; LeDoux, J.M.; Hu, J.; Du, J.
    Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity (2010), J. Biol. Chem., 285, 21249-21257.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
staurosporine in myotubes, staurosporine increases proteasome activity by 38% and stimulates cleavage of proteasome subunits, Rpt2, Rpt6, and Rpn2. Rpt5 and Rpn10 subunits are not cleaved in staurosporine-treated myotubes Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
DEVD-CHO a potent caspase-3 inhibitor Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
19 S proteasome + H2O Mus musculus caspase-3 specifically cleaves the 19 S component of 26 S proteasome Rpt2, Rpt6, and Rpn2 in skeletal muscle stimulating proteasome activity ?
-
?
Rpt2 + H2O Mus musculus
-
?
-
?
Rpt5 + H2O Mus musculus in myoblasts, Rpt5 is sensitive to caspase-3-induced cleavage but in myotubes, it is not sensitive. Caspase-3 cleavage of Rpt5 is responsible for the difference in the patterns of proteasome subunit cleavage in myoblasts compared with myotubes ?
-
?
Rpt6 + H2O Mus musculus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
C2C12 cell
-
Mus musculus
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heart
-
Mus musculus
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myoblast
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Mus musculus
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myotube
-
Mus musculus
-
skeletal muscle during differentiation of myoblasts to myotubes, there is an obligatory, transient increase in caspase-3 activity, accompanied by a corresponding increase in proteasome activity and cleavage of Rpt2 and Rpt6 Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
19 S proteasome + H2O
-
Mus musculus ?
-
?
19 S proteasome + H2O caspase-3 specifically cleaves the 19 S component of 26 S proteasome Rpt2, Rpt6, and Rpn2 in skeletal muscle stimulating proteasome activity Mus musculus ?
-
?
LLVY-4-methylcoumarin 7-amide + H2O
-
Mus musculus LLVY + 7-amino-4-methylcoumarin
-
?
additional information the caspase-3 cleavage site is present within the ATPase domain of Rpt subunits Mus musculus ?
-
?
Rpt2 + H2O
-
Mus musculus ?
-
?
Rpt5 + H2O
-
Mus musculus ?
-
?
Rpt5 + H2O in myoblasts, Rpt5 is sensitive to caspase-3-induced cleavage but in myotubes, it is not sensitive. Caspase-3 cleavage of Rpt5 is responsible for the difference in the patterns of proteasome subunit cleavage in myoblasts compared with myotubes Mus musculus ?
-
?
Rpt6 + H2O
-
Mus musculus ?
-
?

General Information

General Information Comment Organism
malfunction in mice with a muscle wasting condition, chronic kidney disease, occurs cleavage of subunits Rpt2 and Rpt6 and stimulation of proteasome activity Mus musculus
metabolism the stage of muscle cell differentiation determines not only the caspase-3-induced subunit cleavage patterns but also the changes in proteasome activity Mus musculus
physiological function caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity. Caspase-3 increases proteasome activity in myotubes but not in myoblasts by cleaving Rpt2 and Rpt6, while in myoblasts, caspase-3 cleaves Rpt5 to decrease proteasome activity, a feed-forward amplification that augments muscle proteolysis in catabolic conditions, overview Mus musculus