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Literature summary for 3.4.22.56 extracted from

  • Ganesan, R.; Mittl, P.R.; Jelakovic, S.; Gruetter, M.G.
    Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis (2006), J. Mol. Biol., 359, 1378-1388.
    View publication on PubMed
Search for more literature for 3.4.22.56

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitor Asp-Glu-Val-Asp-chloromethylketone Homo sapiens

Protein Variants

Protein Variants Comment Organism
D179A increase both in kcat- and KM-value. D179 is involved in substrate recognition Homo sapiens
DELTA176-181 decrease both in kcat- and KM-value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Asp-Glu-Val-Asp-chloromethylketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 4.75, 37°C Homo sapiens
0.0048
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 4.75, 37°C Homo sapiens
0.0069
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 7.2, 37°C Homo sapiens
0.008
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 4.75, 37°C Homo sapiens
0.0088
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 7.2, 37°C Homo sapiens
0.0097
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
0.0122
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 4.75, 37°C Homo sapiens
0.013
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 4.75, 37°C Homo sapiens
0.0138
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
0.0163
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 4.75, 37°C Homo sapiens
0.0255
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens
0.0335
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P42574 recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, inclusion bodies produced in Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens acetyl-L-Asp-L-Glu-L-Val-L-Asp + 7-amino-4-methylcoumarin
-
 ?
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzoyl-L-Asp-L-Glu-L-Val-L-Asp + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
0.03 0.55 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
0.06
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 4.75, 37°C Homo sapiens
0.08
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 4.75, 37°C Homo sapiens
0.16
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 4.75, 37°C Homo sapiens
0.33
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 4.75, 37°C Homo sapiens
1.1
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 4.75, 37°C Homo sapiens
1.1
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens
1.37
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 4.75, 37°C Homo sapiens
1.43
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 7.2, 37°C Homo sapiens
1.54
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant DELTA176-181, pH 7.2, 37°C Homo sapiens
3.5
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens
6.01
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
7.08
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin wild-type, pH 7.2, 37°C Homo sapiens
19.04
-
 benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens
19.56
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin mutant D179A, pH 7.2, 37°C Homo sapiens