Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no influence of muscle stretching to activation of calpain-3 | Rattus norvegicus |
General Stability | Organism |
---|---|
up to 60 min, in presence of 200 nM Ca2+, no spontaneous autolysation | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | bound to the N2A line on titin or at the triad junctions | Rattus norvegicus | 5737 | - |
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at less than 50 nM Ca2+ calpain-3 remains nonactivated, at 200 nM Ca2+ calpain-3 becomes active | Rattus norvegicus | |
Ca2+ | calpain-3 autolyzes (becoming proteolytically active) in a tightly calcium-dependent manner. It remains in its nonactivated full-length form if [Ca2+] is maintained at up to 50 nM, the normal resting level, even with brief increases to 0.002-0.02 mM during repeated tetanic contractions, but it becomes active (though still bound) if [Ca2+] is kept slightly elevated at 200 nM (about 20% autolysis in 1 h). Calpain-3 does not spontaneously autolyze even when free in solution with 200 nM Ca2+ for up to 60 min | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
Western blot | Rattus norvegicus |
94000 | - |
full-length, unautolyzed form of calpain-3,SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | - |
lang-Evans hooded rats, male, 6-8 month old | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | musculus extensor digitorum longus, musculus soleus | Rattus norvegicus | - |
skeletal muscle | virtually all calpain-3 present in mature muscle fibers is tightly bound in the vicinity of the titin N2A line and triad junctions, most calpain-3 is evidently bound within the contractile filament lattice | Rattus norvegicus | - |
skeletal muscle fiber | - |
Rattus norvegicus | - |
Synonyms | Comment | Organism |
---|---|---|
calpain-3 | - |
Rattus norvegicus |
p94 | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Rattus norvegicus |