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Literature summary for 3.4.22.54 extracted from

  • Murphy, R.M.; Lamb, G.D.
    Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch (2009), J. Biol. Chem., 284, 7811-7819.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.54

Activating Compound

Activating Compound Comment Organism Structure
additional information no influence of muscle stretching to activation of calpain-3 Rattus norvegicus

General Stability

General Stability Organism
up to 60 min, in presence of 200 nM Ca2+, no spontaneous autolysation Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm bound to the N2A line on titin or at the triad junctions Rattus norvegicus 5737
-
soluble
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at less than 50 nM Ca2+ calpain-3 remains nonactivated, at 200 nM Ca2+ calpain-3 becomes active Rattus norvegicus
Ca2+ calpain-3 autolyzes (becoming proteolytically active) in a tightly calcium-dependent manner. It remains in its nonactivated full-length form if [Ca2+] is maintained at up to 50 nM, the normal resting level, even with brief increases to 0.002-0.02 mM during repeated tetanic contractions, but it becomes active (though still bound) if [Ca2+] is kept slightly elevated at 200 nM (about 20% autolysis in 1 h). Calpain-3 does not spontaneously autolyze even when free in solution with 200 nM Ca2+ for up to 60 min Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 Western blot Rattus norvegicus
94000
-
 full-length, unautolyzed form of calpain-3,SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus
-
 lang-Evans hooded rats, male, 6-8 month old
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle musculus extensor digitorum longus, musculus soleus Rattus norvegicus
-
skeletal muscle virtually all calpain-3 present in mature muscle fibers is tightly bound in the vicinity of the titin N2A line and triad junctions, most calpain-3 is evidently bound within the contractile filament lattice Rattus norvegicus
-
skeletal muscle fiber
-
 Rattus norvegicus
-

Synonyms

Synonyms Comment Organism
calpain-3
-
 Rattus norvegicus
p94
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
 Rattus norvegicus