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Literature summary for 3.4.22.53 extracted from

  • Mkwetshana, N.; Naude, R.J.; Oelofsen, W.; Muramoto, K.; Naganuma, T.
    The purification and characterization of m-calpain from ostrich brain (2002), Int. J. Biochem. Cell Biol., 34, 337-347.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
Struthio camelus
leupeptin
-
Struthio camelus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.18
-
succinyl-Leu-Tyr-7-amido-4-methylcoumarin pH 7.5, room temperature Struthio camelus
5.33
-
t-Boc-Leu-Met-methylcoumarin pH 7.5, room temperature Struthio camelus
7.5
-
Boc-Val-Leu-Lys-methylcoumarin pH 7.5, room temperature Struthio camelus
19.32
-
succinyl-Leu-Leu-Val-Tyr-methylcoumarin pH 7.5, room temperature Struthio camelus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM required for optimal activity Struthio camelus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
x * 84000, SDS-PAGE Struthio camelus

Organism

Organism UniProt Comment Textmining
Struthio camelus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Struthio camelus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Struthio camelus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Struthio camelus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Val-Leu-Lys-methylcoumarin + H2O
-
Struthio camelus ?
-
?
casein + H2O
-
Struthio camelus ?
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
Struthio camelus succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
Struthio camelus succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
?
t-Boc-Leu-Met-methylcoumarin + H2O
-
Struthio camelus ?
-
?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Struthio camelus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Struthio camelus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 10°C: about 60% of maximal activity, 40°C: about 45% of maximal activity Struthio camelus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0394
-
succinyl-Leu-Leu-Val-Tyr-methylcoumarin pH 7.5, room temperature Struthio camelus
0.04
-
succinyl-Leu-Tyr-7-amido-4-methylcoumarin pH 7.5, room temperature Struthio camelus
0.341
-
Boc-Val-Leu-Lys-methylcoumarin pH 7.5, room temperature Struthio camelus
0.546
-
t-Boc-Leu-Met-methylcoumarin
-
Struthio camelus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Struthio camelus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity Struthio camelus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00092
-
leupeptin room temperature, pH 7.5, with succinyl-Met-Leu-methylcoumarin as substrate Struthio camelus
0.00206
-
antipain room temperature, pH 7.5, with succinyl-Met-Leu-methylcoumarin as substrate Struthio camelus