Inhibitors | Comment | Organism | Structure |
---|---|---|---|
calpeptin | 50% inhibition of maximal caseinolytic activity at 10 nM and 21 nM for retinal and brain calpain | Bos taurus | |
Ep-475 | 0.001 mM, 49% inhibition of the enzyme from retina, 46% inhibition of the enzyme from brain | Bos taurus | |
leupeptin | 0.001 mM, 67% inhibition of enzyme from retina and brain | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoskeleton | - |
Bos taurus | 5856 | - |
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | half maximal activity for retina and brain enzyme is 0.262 mM and 0.311 mM, maximal activity near 1 mM, no activity in presence of 0.03 mM | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
photoreceptor | outer segment | Bos taurus | - |
retina | highest activity in plexiform layers and in the photoreceptor outer segments. In dark-adapted retinas the label is distributed throughout the outer segments. In light-adapted retinas, outer segment labelling is concentrated in the connecting cilium and the inner segments are labeled | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-actin + H2O | slow degradation | Bos taurus | ? | - |
? | |
casein + H2O | - |
Bos taurus | ? | - |
? | |
additional information | no proteolysis of actin | Bos taurus | ? | - |
? | |
additional information | the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II | Bos taurus | ? | - |
? | |
myosin II heavy chain + H2O | rapid proteolysis | Bos taurus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
enzyme from retina and brain | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, enzyme from retina and brain | Bos taurus |