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Literature summary for 3.4.22.51 extracted from

  • Judice, W.A.; Cezari, M.H.; Lima, A.P.; Scharfstein, J.; Chagas, J.R.; Tersariol, I.L.; Juliano, M.A.; Juliano, L.
    Comparison of the specificity, stability and individual rate constants with respective activation parameters for the peptidase activity of cruzipain and its recombinant form, cruzain, from Trypanosoma cruzi (2001), Eur. J. Biochem., 268, 6578-6586.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine recombinant form without C-terminal extension Trypanosoma cruzi
0.0006
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin recombinant form without C-terminal extension Trypanosoma cruzi
0.001
-
 2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 Trypanosoma cruzi
0.0017
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl Km increases up to 400 mM, then decreases and remains constant at 50-60% up to 1 M, kcat maximum at 300 mM Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q598P5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoic acid-ALRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoic acid-KLGFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O and analogues Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
benzoyl-FR-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0433
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi
0.055
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin recombinant form without C-terminal extension Trypanosoma cruzi
0.0683
-
 2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine recombinant form without C-terminal extension Trypanosoma cruzi
0.08
-
 2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Trypanosoma cruzi