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Literature summary for 3.4.22.49 extracted from

  • Boos, D.; Kuffer, C.; Lenobel, R.; Koerner, R.; Stemmann, O.
    Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase (2008), J. Biol. Chem., 283, 816-823.
    View publication on PubMed
Search for more literature for 3.4.22.49

Protein Variants

Protein Variants Comment Organism
S1126A a doxycycline-induced overexpression of S1126A mutant in HEK-293 cells leads to an increase of the G2/M cell population from 24 to 49% within 24 h compared to wild-type. Giemsa-stained prometaphase-chromosomes from S1126A expressing cells are mostly separated (61%), whereas those from wild-type or T1346E/T1363E/S1399D expressing cells are almost always paired (78 and 83%, respectively) Homo sapiens
T1346A/T1363A/S1399A this triple mutant binds relatively more Cdk1 than S1126A but its Cdk1 binding capacity is much decreased compared to wildtype. A doxycycline-induced overexpression of the triple mutant in HEK-293 cells leads to an increase of the G2/M cell population from 24 to 37% within 24 h compared to wild-type. Giemsa-stained prometaphase-chromosomes from triple mutant expressing cells are mostly separated (61%), whereas those from wild-type or T1346E/T1363E/S1399D expressing cells are almost always paired (78 and 83%, respectively) Homo sapiens
T1346A/T1363A/S1399D the mitotic arrest phenotype caused by the T1346A/T1363A/S1399A triple mutant is largely abrogated when just residue 1399 is changed to aspartate. Thus among the phosphorylation sites within the Cdc6-like domain serine 1399 is sufficient to support Cdk1-dependent inhibition of separase Homo sapiens
T1346E/T1363E/S1399D changing the phosphorylation sites within the Cdc6-like domain to acidic residues results in a separase that can still be inhibited by Cdk1 in vivo Homo sapiens
T1346E/T1363E/S1399S the mitotic arrest phenotype caused by the T1346A/T1363A/S1399A triple mutant is largely abrogated when just residue 1399 is changed to serine. Thus among the phosphorylation sites within the Cdc6-like domain serine 1399 is sufficient to support Cdk1-dependent inhibition of separase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cdk1 kinase phosphorylation-dependent binding of the cyclin B1 subunit of Cdk1 kinase to a Cdc6-like domain of separase inhibits separase Homo sapiens
securin
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein three phoshorylation sites within the the Cdc6-like domain of separase are identified which are critical for Cdk1 binding: Thr 1346, Thr 1363 and Ser 1399 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
separase is expressed as a GST- or myc-tagged fusion protein in Escherichia coli BL-21 and HEK-293 cells Homo sapiens

Synonyms

Synonyms Comment Organism
separase
-
 Homo sapiens