Literature summary for 3.4.22.47 extracted from

Fujimura, S.; Hirai, K.; Shibata, Y.; Nakayama, K.; Nakamura, T.
Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis (1998), FEMS Microbiol. Lett., 163, 173-179.

Search for more literature for 3.4.22.47

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	30 mM, 197% increase in activity	Porphyromonas gingivalis
cysteine	3 mM, 166% increase in activity	Porphyromonas gingivalis
dithiothreitol	30 mM, 315% increase in activity	Porphyromonas gingivalis
EDTA	2 mM, 79% increase in activity	Porphyromonas gingivalis
EGTA	2 mM, 79% increase in activity	Porphyromonas gingivalis

Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	2 mM, 65% inhibition	Porphyromonas gingivalis
p-hydroxymercuribenzoate	0.2 mM, 68% inhibition	Porphyromonas gingivalis
tosyl-L-lysine chloromethyl ketone	0.05 mM, 81% inhibition	Porphyromonas gingivalis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell envelope	-	Porphyromonas gingivalis	30313	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	x * 48000, SDS-PAGE	Porphyromonas gingivalis

Organism

Organism	UniProt	Comment	Textmining
Porphyromonas gingivalis	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture supernatant	-	Porphyromonas gingivalis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O	7% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide	Porphyromonas gingivalis	benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O	-	?
D-Val-Phe-Lys-p-nitroanilide + H2O	70% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide	Porphyromonas gingivalis	D-Val-Phe-Lys + p-nitroaniline	-	?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O	-	Porphyromonas gingivalis	N-p-tosyl-Gly-Pro-Lys + p-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
?	x * 48000, SDS-PAGE	Porphyromonas gingivalis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	68% loss of activity after 10 min, 97% loss of activity after 30 min	Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Porphyromonas gingivalis

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Release 2023.1 (January 2023)