Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Foot-and-mouth disease virus |
expressed in Escherichia coli BL21(DE3)pLysS cells | porcine reproductive and respiratory syndrome virus |
Protein Variants | Comment | Organism |
---|---|---|
C51A | inactive | Foot-and-mouth disease virus |
C51A | inactive | porcine reproductive and respiratory syndrome virus |
L200F | the mutant is impaired in activity but still allows self-processing | porcine reproductive and respiratory syndrome virus |
L200F | the mutant is impaired in but still allows self-processing | Foot-and-mouth disease virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | self-processing inactivates leader proteinase nsp1alpha | porcine reproductive and respiratory syndrome virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19100 | - |
2 * 19100, calculated from amino acid sequence | Foot-and-mouth disease virus |
19100 | - |
2 * 19100, calculated from amino acid sequence | porcine reproductive and respiratory syndrome virus |
28800 | - |
2 * 28800, SDS-PAGE | Foot-and-mouth disease virus |
28800 | - |
2 * 28800, SDS-PAGE | porcine reproductive and respiratory syndrome virus |
41400 | - |
gel filtration | Foot-and-mouth disease virus |
41400 | - |
gel filtration | porcine reproductive and respiratory syndrome virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
eukaryotic initiation factor eIF4G + H2O | Foot-and-mouth disease virus | - |
? | - |
? | |
eukaryotic initiation factor eIF4G + H2O | porcine reproductive and respiratory syndrome virus | - |
? | - |
? | |
additional information | Foot-and-mouth disease virus | the enzyme cleaves itself off the nascent viral polyprotein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Foot-and-mouth disease virus | - |
- |
- |
porcine reproductive and respiratory syndrome virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitatio, Mono Q column chromatography, and Superdex 75 gel filtration | porcine reproductive and respiratory syndrome virus |
ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 75 gel filtration | Foot-and-mouth disease virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eukaryotic initiation factor eIF4G + H2O | - |
Foot-and-mouth disease virus | ? | - |
? | |
eukaryotic initiation factor eIF4G + H2O | - |
porcine reproductive and respiratory syndrome virus | ? | - |
? | |
additional information | the enzyme cleaves itself off the nascent viral polyprotein | Foot-and-mouth disease virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 19100, calculated from amino acid sequence | Foot-and-mouth disease virus |
homodimer | 2 * 19100, calculated from amino acid sequence | porcine reproductive and respiratory syndrome virus |
homodimer | 2 * 28800, SDS-PAGE | Foot-and-mouth disease virus |
homodimer | 2 * 28800, SDS-PAGE | porcine reproductive and respiratory syndrome virus |
Synonyms | Comment | Organism |
---|---|---|
Lbpro | - |
Foot-and-mouth disease virus |
Leader proteinase | - |
Foot-and-mouth disease virus |
Leader proteinase | - |
porcine reproductive and respiratory syndrome virus |
nsp1alpha | - |
porcine reproductive and respiratory syndrome virus |