Cloned (Comment) | Organism |
---|---|
cloning of NIa in Escherichia coli, overexpression of HA-tagged NIa in rat B103 neuroblastoma cells | turnip mosaic virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol | turnip mosaic virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | does not completely inhibit NIa activity | turnip mosaic virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | predominantly | turnip mosaic virus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
turnip mosaic virus | - |
TuMV | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid-beta peptide + H2O | degradation of oligomeric as well as monomeric forms of Amyloid-beta, presence of the consensus sequence, Val12-His-His-Gln15, near the presumptive alpha-secretase cleavage site of the amyloid-beta peptide | turnip mosaic virus | ? | - |
? | |
additional information | NIa possesses a relatively strict substrate specificity with a preference for Val-Xaa-His-GlnQ, with the scissile bond located after Gln | turnip mosaic virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NIa protease | - |
turnip mosaic virus |
nuclear inclusion a protease | - |
turnip mosaic virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | turnip mosaic virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | turnip mosaic virus |
General Information | Comment | Organism |
---|---|---|
additional information | overexpression of NIa in rat B103 neuroblastoma cells results in a significant reduction in cell death caused by both intracellularly generated and exogenously added Amyloidbeta. Moreover, lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice significantly reduces the levels of Amyloid-beta and plaques in the brain | turnip mosaic virus |
physiological function | the nuclear inclusion a protease of turnip mosaic virus is responsible for the processing of the viral polyprotein into functional proteins. Degradation of Amyloid-beta in the cytoplasm can be a novel strategy to control the levels of Amyloid-beta, plaque formation, and the associated cell death | turnip mosaic virus |