Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C73A | inactive mutant | Homo sapiens |
H369A | inactive mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Arg-Ala | 86% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
Cd2+ | 5% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
Cu2+ | 9% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
E64 | 15% inhibition of HLTase activity at 0.012 mM, 97% at 0.125 mM | Homo sapiens | |
E64 | 99% inhibition of HLTase activity at 0.012 mM | Saccharomyces cerevisiae | |
H2O2 | 41% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
iodoacetamide | complete inhibition of HLTase activity at 2 mM | Homo sapiens | |
iodoacetamide | 94% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
Leu-Ala | 50% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
Lys-Ala | 77% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
Lys-Leu | 26% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
Zn2+ | 70% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Homo sapiens | 5622 | - |
intracellular | - |
Saccharomyces cerevisiae | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | ? | - |
? | |
additional information | Saccharomyces cerevisiae | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
several strains | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Homo sapiens |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | Homo sapiens | ? | - |
? | |
additional information | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BLH | - |
Homo sapiens |
Hcy-thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
Hcy-thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
homocysteine thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
homocysteine thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
HTLase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
HTLase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
yBLH | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Saccharomyces cerevisiae |