Literature summary for 3.4.22.38 extracted from

McQueney, M.S.; Amegadzie, B.Y.; D'Alessio, K.; Hanning, C.R.; McLaughlin, M.M.; McNulty, D.; Carr, S.A.; Ijames, C.; Kurdyla, J.; Jones, C.S.
Autocatalytic activation of human cathepsin K (1997), J. Biol. Chem., 272, 13955-13960.

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Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23590	-	electrospray mass spectrometry of Ala115-terminated enzyme	Homo sapiens
23650	-	electropspray mass spectrometry of Arg114-terminated enzyme	Homo sapiens
35300	-	procathepsin K, calculation from amino acid sequence, procathepsin K is autocatalytically activated at 4 C, pH 4, the activation is enhanced by 1% mature cathepsin K, cleavage of procathepsin K after Ala15	Homo sapiens
36370	-	procathepsin K, MALDI-Mass spectromety, procathepsin K is autocatalytically activated at 4 C, pH 4, the activation is enhanced by 1% mature cathepsin K, cleavage of procathepsin K after Ala15	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	recombinant enzymes, expressed in Sf21 cells, baculovirus-infected, wild-type and mutant enzyme in which Cys139 is changed to Ser	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	sugars comprise 3% of the enzyme mass, Asn103 is the only site of glycosylation	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.007	0.014	substrate benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide, procathepsin K	Homo sapiens
15	25	substrate benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide, mature enzyme	Homo sapiens

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Release 2023.1 (January 2023)