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Literature summary for 3.4.22.37 extracted from

  • Skottrup, P.D.; Leonard, P.; Kaczmarek, J.Z.; Veillard, F.; Enghild, J.J.; OKennedy, R.; Sroka, A.; Clausen, R.P.; Potempa, J.; Riise, E.
    Diagnostic evaluation of a nanobody with picomolar affinity toward the protease RgpB from Porphyromonas gingivalis (2011), Anal. Biochem., 415, 158-167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.37

Application

Application Comment Organism
biotechnology a naive camel nanobody library is constructed and phage display is used to select one nanobody toward RgpB with picomolar affinity. The nanobody is highly specific for RgpB given that it does not bind to the homologous gingipain HRgpA, indicating the presence of a binding epitope within the immunoglobulin-like domain of RgpB. RgpB can be used as a specific biomarker for Porphyromonas gingivalis infection Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment) Organism
RgpB with the C-terminal His-tag is produced by the Porphyromonas gingivalis W83 strain bearing the modified rgpB gene Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity and ion exchange chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
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 Porphyromonas gingivalis Nalpha-benzoyl-L-Arg + 4-nitroaniline
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 ?

Synonyms

Synonyms Comment Organism
RgpB
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 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Porphyromonas gingivalis