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Literature summary for 3.4.22.37 extracted from

  • Chen, Z.; Potempa, J.; Polanowski, A.; Wikstrom, M.; Travis, J.
    Purification and characterization of a 50-kDa cysteine proteinase (gingipain) from Porphyromonas gingivalis (1992), J. Biol. Chem., 267, 18896-18901.
    View publication on PubMed
Search for more literature for 3.4.22.37

Activating Compound

Activating Compound Comment Organism Structure
cysteine required for full amidolytic activity Porphyromonas gingivalis

General Stability

General Stability Organism
Ca2+ stabilizes Porphyromonas gingivalis
Irreversible loss of activity during lyophilization Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
EDTA completely reversed by addition of excess Ca2+ Porphyromonas gingivalis
EGTA completely reversed by addition of excess Ca2+ Porphyromonas gingivalis
iodoacetamide
-
 Porphyromonas gingivalis
iodoacetic acid
-
 Porphyromonas gingivalis
additional information resistant to inhibition by proteinase inhibitors in human plasma Porphyromonas gingivalis
N-Chlorosuccinimide
-
 Porphyromonas gingivalis
NEM
-
 Porphyromonas gingivalis
p-aminobenzamidine
-
 Porphyromonas gingivalis
tosyl-L-Lys chloromethyl ketone i.e. TLCK Porphyromonas gingivalis
tosyl-L-Phe chloromethyl ketone i.e. TPCK Porphyromonas gingivalis
ZnCl2
-
 Porphyromonas gingivalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ may play a role in enzyme catalysis Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 Porphyromonas gingivalis, gel filtration Porphyromonas gingivalis
50000
-
 1 * 50000, Porphyromonas gingivalis, SDS-PAGE Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Porphyromonas gingivalis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Porphyromonas gingivalis
-

Storage Stability

Storage Stability Organism
-20°C, stable for several months Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Arg + 4-nitroaniline
-
 ?
Benzoyl-Ile-Glu-(gamma-ornithyl)-Gly-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
D-Ile-Pro-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
D-Phe-Pip-Arg 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?
Insulin B-chain + H2O specific cleavage of Arg-+- Porphyromonas gingivalis ?
-
 ?
Mellitin + H2O specific cleavage of Arg-+- Porphyromonas gingivalis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, Porphyromonas gingivalis, SDS-PAGE Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in presence of glycylglycine Porphyromonas gingivalis