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Literature summary for 3.4.22.34 extracted from

  • Vidmar, R.; Vizovisek, M.; Turk, D.; Turk, B.; Fonovic, M.
    Protease cleavage site fingerprinting by label-free in-gel degradomics reveals pH-dependent specificity switch of legumain (2017), EMBO J., 36, 2455-2465 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.34

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
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 Homo sapiens 5764
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q99538
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O the canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp Homo sapiens benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Ala-Ala-Asp-7-amido-4-methylcoumarin + H2O the canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp Homo sapiens benzyloxycarbonyl-Ala-Ala-Asp + 7-amino-4-methylcoumarin
-
 ?
additional information a gel based label-free proteomic approach (DIPPS-direct in-gel profiling of protease specificity) is developed that enables quick and reliable determination of protease cleavage specificities under large variety of experimental conditions. The methodology is based on in-gel digestion of the gel-separated proteome with the studied protease, enrichment of cleaved peptides by gel extraction, and subsequent mass spectrometry analysis combined with a length-limited unspecific database search. Specificity profiling of legumain at pH 4.0 reveales a pH-dependent change in the specificity of this protease. The canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp Homo sapiens ?
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 ?

General Information

General Information Comment Organism
physiological function potential role of legumain in differential substrate recognition during endolysosomal maturation Homo sapiens