Literature summary for 3.4.22.34 extracted from

Smith, R.; Johansen, H.T.; Nilsen, H.; Haugen, M.H.; Pettersen, S.J.; Maelandsmo, G.M.; Abrahamson, M.; Solberg, R.
Intra- and extracellular regulation of activity and processing of legumain by cystatin E/M (2012), Biochimie, 94, 2590-2599.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HEK-293 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cystatin E/M	cystatin E/M controls both intra- and extracellular legumain activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome	-	Homo sapiens	5768	-
lysosome	-	Homo sapiens	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, pro-enzyme form, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Homo sapiens	-
liver	-	Homo sapiens	-
placenta	-	Homo sapiens	-
spleen	-	Homo sapiens	-
testis	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 46000-47000, active enzyme form, SDS-PAGE	Homo sapiens
?	x * 56000, pro-enzyme form, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
AEP	-	Homo sapiens
asparaginyl endopeptidase,	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	secretion of pro-legumain is inhibited by treatment with brefeldin A	down
Homo sapiens	bafilomycin A1 enhances the secretion of the the enzyme, which is also is up-regulated in tumour and tumour-associated cells	up

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Release 2023.1 (January 2023)