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Literature summary for 3.4.22.34 extracted from
- Blastocystis legumain is localized on the cell surface, and specific inhibition of its activity implicates a pro-survival role for the enzyme (2010), J. Biol. Chem., 285, 1790-1798.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| glutathione S-transferase-fusion protein expressed in Escherichia coli BL21(DE3) | Blastocystis sp. BW-2009a |
| glutathione S-transferase-fusion protein expressed in Escherichia coli BL21(DE3) | Blastocystis hominis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carbobenzyloxy-Ala-Ala-(aza-Asn)-epoxycarboxylate ethyl ester | - |
Blastocystis hominis |  |
| carbobenzyloxy-Ala-Ala-(aza-Asn)-epoxycarboxylate ethyl ester | - |
Blastocystis sp. BW-2009a | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, 2-D electrophoresis | Blastocystis sp. BW-2009a |
| 30000 | - |
x * 30000, 2-D electrophoresis | Blastocystis hominis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastocystis hominis | D1M8Z4 | - |
- |
| Blastocystis sp. BW-2009a | C1K3M8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography (glutathione-Sepharose) | Blastocystis sp. BW-2009a |
| affinity chromatography (glutathione-Sepharose) | Blastocystis hominis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Blastocystis sp. BW-2009a | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Blastocystis hominis | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, 2-D electrophoresis | Blastocystis sp. BW-2009a |
| ? | x * 30000, 2-D electrophoresis | Blastocystis hominis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| legumain | - |
Blastocystis sp. BW-2009a |
| legumain | - |
Blastocystis hominis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
biphasic pH optima with peaks at pH 4 (about 70% activity) and pH 7.5 (100% activity) | Blastocystis sp. BW-2009a |
| 7.5 | - |
biphasic pH optima with peaks at pH 4 (about 70% activity) and pH 7.5 (100% activity) | Blastocystis hominis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | - |
Blastocystis sp. BW-2009a |
| 3.5 | 8 | - |
Blastocystis hominis |
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