Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | expression levels of endogenous legumain elevate in HEK-293 cells following stress | Homo sapiens |
Application | Comment | Organism |
---|---|---|
drug development | enzyme serves as a promising candidate for prodrug tumor therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
embryonic kidney cells are stably transfected with human legumain to achieve overexpression in vitro, HEK-Leg cells expressed both active and inactive legumain and secreted it to the medium | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
mature, active form, determined by SDS-PAGE and Westen blot analysis | Homo sapiens |
46000 | - |
non-mature, active form, determined by SDS-PAGE and Westen blot analysis | Homo sapiens |
56000 | - |
inactive proform, determined by SDS-PAGE and Westen blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme promote cell migration and is associated with enhanced tissue invasion and metastases | ? | - |
? | |
additional information | Homo sapiens | legumain is a member of the C13 family of peptidases that specifically cleaves asparaginyl bonds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | recombinant legumain | Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
HEK-293 serum-starved cells show a slight 1.2fold increase in enzymatic activity toward carbobenzyloxy-Ala-Ala-Asn-amido-4-methyl coumarin compared to that of nonstarved 293 HEK cells, while PC-3 serum-starved or nonstarved cells shows no significant difference in enzymatic activity | Homo sapiens |
additional information | - |
HEK-293-overexpressing legumain cells cleave 100% of the prodrug carbobenzyloxy-Ala-Ala-Asn-ethylenediamine-etoposide, whereas HEK-293 cells, which express low levels of legumain, cleave only 33% of it | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbobenzyloxy-Ala-Ala-Asn-ethylenediamine-etoposide + H2O | legumain releases the chemotherapeutic agent etoposide, as the active drug | Homo sapiens | etoposide-ethylenediamine + carbobenzyloxy-Ala-Ala-Asn | - |
? | |
CBZ-Ala-Ala-Asn-amido-4-methylcoumarin + H2O | - |
Homo sapiens | CBZ-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
additional information | enzyme promote cell migration and is associated with enhanced tissue invasion and metastases | Homo sapiens | ? | - |
? | |
additional information | legumain is a member of the C13 family of peptidases that specifically cleaves asparaginyl bonds | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
legumain | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant legumain cleaves carbobenzyloxy-Ala-Ala-Asn-amido-4-methyl coumarin more potently at pH 6.5 than at pH 5.0 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
no enzymatic activity at pH 7.0, legumain undergoes denaturation at these basic conditions | Homo sapiens |