Literature summary for 3.4.22.34 extracted from

Jackson, M.A.; Rae, A.L.; Casu, R.E.; Grof, C.P.; Bonnett, G.D.; Maclean, D.J.
A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole (2007), Funct. Plant Biol., 34, 633-644.

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Application

Application	Comment	Organism
biotechnology	studies on vacuolar targeting, targeting motif of legumain as a potential tool for plant engineering analyzed	Saccharum officinarum

Cloned(Commentary)

Cloned (Comment)	Organism
in Escherichia coli, construct pCvsEndoexp1 transformed into callus derived from Q117 meristematic tissue, transgenic plants regenerated	Saccharum officinarum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	vacuoles within the sugarcane storage parenchyma tissue, bioinformatical studies on vacuolar targeting, GFP-constructs sucessfully targeted to lytic vacuolar compartments of Saccharum officinarum	Saccharum officinarum	5773	-

Organism

Organism	UniProt	Comment	Textmining
Saccharum officinarum	Q0ZHB0	encoded by VPE-1 gene; sugarcane, transgenic plants regenerated from callus culture	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	C-terminus, in endogenous and in recombinant protein	Saccharum officinarum

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli, SDS-PAGE	Saccharum officinarum

Source Tissue

Source Tissue	Comment	Organism	Textmining
callus	for generation of transformed plants, GFP reporter analysis indicated	Saccharum officinarum	-
whole plant	control plants and transformed plants, GFP reporter analysis indicated	Saccharum officinarum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	cleavage sites for N- and C-terminus indicated, predicted motif for vacuolar proteins analyzed, targeting ability sucessfully tested in Saccharum officinarum and in other plant species by transient expression analysis after biolistic bombardment using the construct pCvsEndoexp1	Saccharum officinarum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	bioinformatic approach, studies on vacuolar targeting by determination of minimal vacuole targeting motifs, GFP reporter analysis and subcellular staining indicated	Saccharum officinarum	?	-	?
additional information	bioinformatic approach, studies on vacuolar targeting, determination of minimal vacuole targeting motifs, GFP reporter analysis and subcellular staining indicated	Saccharum officinarum	?	-	?

Synonyms

Synonyms	Comment	Organism
Asparaginyl endopeptidase	-	Saccharum officinarum

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Release 2023.1 (January 2023)