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Literature summary for 3.4.22.34 extracted from
- Mammalian legumain (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1302-1305.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme requires activation by a thiol compound | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence and structure determination and analysis, expression in mammalian cells | Mus musculus |
| gene PRSC1, DNA and amino acid sequence determination and analysis, localization on chromosome 14q32.1, expression in insect cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of null-legumain mice | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystatin | from chicken egg white | Sus scrofa | |
| cystatin C | human | Sus scrofa | |
| iodoacetamide | - |
Sus scrofa |  |
| iodoacetate | - |
Sus scrofa | |
| additional information | no inhibition by E64 and leupeptin | Sus scrofa | |
| N-ethylmaleimide | - |
Sus scrofa | |
| N-Phenylmaleimide | strong inhibition | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Mus musculus | 5764 | - |
| lysosome | - |
Homo sapiens | 5764 | - |
| lysosome | - |
Sus scrofa | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme | Homo sapiens |
| 49000 | - |
x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the inhibition of osteoclast formation and bone resorption is a nonenzymatic function of prolegumain | ? | - |
? | |
| neurotensin + H2O | Sus scrofa | specific cleavage | ? | - |
? | |
| progelatinase A + H2O | Homo sapiens | activation | gelatinase A + gelatinase A propeptide | - |
? | |
| Vasoactive intestinal peptide + H2O | Sus scrofa | specific cleavage | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
| glycoprotein | N-glycosylation, deglycosylation by N-glycosidase F | Sus scrofa |
| proteolytic modification | autoactivation/maturation by cleavage at Asn323 | Homo sapiens |
| proteolytic modification | autoactivation/maturation by cleavage at Asn323 | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from kidney, overview | Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | the enzyme contains essential catalytic His and Cys residues | Mus musculus | |
| hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | the enzyme contains essential catalytic His and Cys residues | Homo sapiens | |
| hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | the enzyme contains essential catalytic His and Cys residues | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified native enzyme from kidney, pH 5.8, little loss of activity over several months | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Boc-Asn-4-nitrophenyl ester + H2O | - |
Homo sapiens | Boc-Asn + 4-nitrophenol | - |
? | |
| additional information | the inhibition of osteoclast formation and bone resorption is a nonenzymatic function of prolegumain | Homo sapiens | ? | - |
? | |
| additional information | the enzyme acts strictly as asparaginyl endopeptidase, substrate specificity, overview | Sus scrofa | ? | - |
? | |
| neurotensin + H2O | specific cleavage | Sus scrofa | ? | - |
? | |
| progelatinase A + H2O | - |
Homo sapiens | gelatinase A + gelatinase A propeptide | - |
? | |
| progelatinase A + H2O | activation | Homo sapiens | gelatinase A + gelatinase A propeptide | - |
? | |
| Vasoactive intestinal peptide + H2O | specific cleavage | Sus scrofa | ? | - |
? | |
| Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Asparaginyl endopeptidase | - |
Mus musculus |
| Asparaginyl endopeptidase | - |
Homo sapiens |
| Asparaginyl endopeptidase | - |
Sus scrofa |
| More | the enzyme belongs to the C13 peptidase family | Mus musculus |
| More | the enzyme belongs to the C13 peptidase family | Homo sapiens |
| More | the enzyme belongs to the C13 peptidase family | Sus scrofa |
| osteoclast inhibitory peptide 2 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | stable, irreversible denaturation at higher pH | Sus scrofa |
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