Cloned (Comment) | Organism |
---|---|
stable overexpression in HEK 293 cells and in C13 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin | - |
Sus scrofa | |
additional information | no inhibition by PMSF, EDTA and 1,10-phenanthroline | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progelatinase A + H2O | Homo sapiens | in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase | gelatinase A + ? | 62 kDa mature form | ? | |
protein + H2O | Sus scrofa | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Sus scrofa | - |
purified enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the 56 kDa proform is cleavaed to the active 46 kDa and 36 kDa forms | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
additional information | no activity in native HEK 293 cell | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gelatinase + H2O | 62 kDa active form, cleavage site is Asn430 | Homo sapiens | gelatinase fragment | 36 kDa active form | ? | |
gelatinase + H2O | 62 kDa active form, cleavage site is Asn430 | Sus scrofa | gelatinase fragment | 36 kDa active form | ? | |
additional information | no activity with pro-/gelatinase B | Homo sapiens | ? | - |
? | |
additional information | no activity with pro-/gelatinase B | Sus scrofa | ? | - |
? | |
progelatinase A + H2O | 72 kDa human proform, activation by cleavage of an asparaginyl peptide bonde to form the N-terminus of the mature active gelatinase | Sus scrofa | gelatinase + ? | 62 kDa mature form | ? | |
progelatinase A + H2O | 72 kDa proform, activation by cleavage of an asparaginyl peptide bonde to form the N-terminus of the mature active gelatinase | Homo sapiens | gelatinase + ? | 62 kDa mature form | ? | |
progelatinase A + H2O | in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase | Homo sapiens | gelatinase A + ? | 62 kDa mature form | ? | |
protein + H2O | - |
Sus scrofa | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lysosomal cysteine proteinase | - |
Homo sapiens |
lysosomal cysteine proteinase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | Homo sapiens |
5.8 | - |
aasy at | Sus scrofa |