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Literature summary for 3.4.22.33 extracted from

  • Das, S.; Bhattacharyya, D.
    Destabilization of human insulin fibrils by peptides of fruit bromelain derived from Ananas comosus (pineapple) (2017), J. Cell. Biochem., 118, 4881-4896 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Ananas comosus O23791
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from ripe fruits by lyophilization and gel filtration Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Ananas comosus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Ananas comosus

General Information

General Information Comment Organism
physiological function small peptides are separated from larger peptides and proteins by fruit bromelain in the human digestive tract, the peptides are capable of destabilizing and desaggregating insulin aggregates (e.g. B23-B30 des-octapeptide) to oligomers. The peptides GG and AAA serving as negative controls show no potency in destabilization of aggregates. Disaggregation potency of the peptides wis also observed when insulin is deposited on Hep-G2 liver cells where no formation of toxic oligomers occurs. Deposition of insulin aggregates in human body leads to dysfunctioning of several organs. Amyloidogenic des-octapeptide (B23-B30 of insulin) incapable of cell signaling also show cytotoxicity similar to insulin. This toxicity can also be neutralized by bromelain derived peptides. Disaggregated insulin has a structure distinctly different from that of its hexameric (native) or monomeric states, FT-IR and far-UV circular dichroism analysis. Ability of auto digested peptides from bromelain to act as an inhibitor of amyloid formation Ananas comosus