BRENDA - Enzyme Database show
show all sequences of 3.4.22.33

An inhibitive determination method for heavy metals using bromelain, a cysteine protease

Shukor, M.Y.; Masdor, N.; Baharom, N.A.; Jamal, J.A.; Abdullah, M.P.; Shamaan, N.A.; Syed, M.A.; Appl. Biochem. Biotechnol. 144, 283-291 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
inhibits casein degradation, complete inhibition
Ananas comosus
Hg2+
inhibits casein degradation, complete inhibition
Ananas comosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
poor or no inhibition by ions K+, Ca2+, Mg2+, Mn2+, Co2+, Bo3+, Al3+, Cr2+, Ni2+, Sn2+, Ag+, Fe2+, Cd2+, Zn2+, W3+, Se2+, As3+, and Pb2+, overview
Ananas comosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ananas comosus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Ananas comosus
-
fruit
bromelain is a mixture of bromelain A and B, the proteolytic enzymes of pineapple fruit
Ananas comosus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
azocasein + H2O
-
695660
Ananas comosus
?
-
-
-
?
benzoyl-L-arginine ethyl ester + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
casein + H2O
enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+
695660
Ananas comosus
?
-
-
-
?
additional information
bromelain is a cysteine protease containing a single thiol group in the active sites
695660
Ananas comosus
?
-
-
-
-
N-benzoyl-L-tyrosine ethyl ester + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
inhibits casein degradation, complete inhibition
Ananas comosus
Hg2+
inhibits casein degradation, complete inhibition
Ananas comosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
poor or no inhibition by ions K+, Ca2+, Mg2+, Mn2+, Co2+, Bo3+, Al3+, Cr2+, Ni2+, Sn2+, Ag+, Fe2+, Cd2+, Zn2+, W3+, Se2+, As3+, and Pb2+, overview
Ananas comosus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Ananas comosus
-
fruit
bromelain is a mixture of bromelain A and B, the proteolytic enzymes of pineapple fruit
Ananas comosus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
azocasein + H2O
-
695660
Ananas comosus
?
-
-
-
?
benzoyl-L-arginine ethyl ester + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
casein + H2O
enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+
695660
Ananas comosus
?
-
-
-
?
additional information
bromelain is a cysteine protease containing a single thiol group in the active sites
695660
Ananas comosus
?
-
-
-
-
N-benzoyl-L-tyrosine ethyl ester + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O
artificial substrate
695660
Ananas comosus
?
-
-
-
?
Other publictions for EC 3.4.22.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731210
Arshad
Bromelain: an overview of indu ...
Ananas comosus
Appl. Microbiol. Biotechnol.
98
7283-7297
2014
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717050
Liang
-
Study on the stability of frui ...
Ananas comosus
Adv. Mater. Res.
421
19-22
2012
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731843
Corzo
-
Pineapple fruit bromelain affi ...
Ananas comosus
Food Chem.
133
631-635
2012
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731908
Devi
-
Pineapple fruit bromelain and ...
Ananas comosus
Indian J. Agric. Biochem.
25
1-7
2012
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717487
Ferreira
-
Purification of bromelain from ...
Ananas comosus
Chem. Eng. Transact.
24
931-936
2011
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717625
Yin
Preparative purification of br ...
Ananas comosus
Food Chem.
129
925-932
2011
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718411
Gautam
-
Comparative study of extractio ...
Ananas comosus
Thai J. Pharm. Sci.
34
67-76
2010
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710318
Neuteboom
An extended AE-rich N-terminal ...
Ananas comosus
Plant Physiol.
151
515-527
2009
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695660
Shukor
An inhibitive determination me ...
Ananas comosus
Appl. Biochem. Biotechnol.
144
283-291
2008
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681047
Hemavathi
-
Reverse micellar extraction of ...
Ananas comosus
J. Chem. Technol. Biotechnol.
82
985-992
2007
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682716
Cabral
Preliminary functional charact ...
Bromelia fastuosa
Protein Pept. Lett.
13
83-89
2006
4
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8
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665208
Hale
Proteinase activity and stabil ...
Ananas comosus
Int. Immunopharmacol.
5
783-793
2005
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665083
Rowan
-
Fruit bromelain ...
Ananas comosus
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1137-1138
2004
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650803
Maurer
Bromelain: biochemistry, pharm ...
Ananas comosus
Cell. Mol. Life Sci.
58
1234-1245
2001
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2
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30296
Suh
-
Purification and characterizat ...
Ananas comosus
Han'guk Nonghwa Hakhoechi
35
300-307
1992
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30279
Rowan
Inhibition of cysteine protein ...
Ananas comosus
FEBS Lett.
269
328-330
1990
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30278
Ota
Reinvestigation of fractionati ...
Ananas comosus
J. Biochem.
98
219-228
1985
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30274
Murachi
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
45
475-485
1976
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30299
Yamada
Purification and characterizat ...
Ananas comosus
J. Biochem.
79
1223-1234
1976
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30301
Toro-Goyco
Immunochemical studies on ping ...
Ananas comosus, Bromelia pinguin
Arch. Biochem. Biophys.
175
359-366
1976
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30300
Sasaki
Antigenic determinant common t ...
Ananas comosus
J. Biochem.
74
635-637
1973
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30277
Ota
Fractionation and some propert ...
Ananas comosus
J. Biochem.
71
817-830
1972
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30273
Murachi
-
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
19
273-284
1970
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1
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1
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1
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1
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3
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1
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1
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