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Literature summary for 3.4.22.33 extracted from

  • Ota, S.; Horie, K.; Hagino, F.; Hashimoto, C.; Date, H.
    Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant (1972), J. Biochem., 71, 817-830.
    View publication on PubMed
Search for more literature for 3.4.22.33

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 Nalpha-benzoyl-Arg amide fruit bromelain C Ananas comosus
4
-
 Nalpha-benzoyl-Arg amide fruit bromelain A and B Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 Ananas comosus, fruit bromelain A, gel filtration Ananas comosus
19000
-
 Ananas comosus, fruit bromelain C, gel filtration Ananas comosus
28000
-
 Ananas comosus, fruit bromelain B, gel filtration Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
fractionation Ananas comosus
fruit bromelain A, B and C Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
Nalpha-Benzoyl-Arg amide + H2O
-
 Ananas comosus Nalpha-Benzoyl-Arg + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0094
-
 Nalpha-benzoyl-Arg amide fruit bromelain C Ananas comosus
0.027
-
 Nalpha-benzoyl-Arg amide fruit bromelain B Ananas comosus
0.033
-
 Nalpha-benzoyl-Arg amide fruit bromelain A Ananas comosus