Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.32 extracted from

  • Gupta, P.; Saleemuddin, M.
    Bioaffinity based oriented immobilization of stem bromelain (2006), Biotechnol. Lett., 28, 917-922.
    View publication on PubMed
Search for more literature for 3.4.22.32

General Stability

General Stability Organism
enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A is more stable to thermal inactivation than native enzyme or enzyme covalently coupled to the CNBr-activated Sepharose Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-for casein is 1.1 mg/ml for the native enzyme, 2 mg/ml for the enzyme covalently coupled to the CNBr-activated Sepharose and 0.54 mg/ml for enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains a single oligosaccharide chain attached to the polypeptide Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 100 min, native enzyme loses 80% of initial activity, enzyme covalently coupled to the CNBr-activated Sepharose loses 70% of the initial activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A loses 50% of initial activity Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 native enzyme, enzyme covalently coupled to the CNBr-activated Sepharose and enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A Ananas comosus

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5.0: native enzyme shows about 25% of maximal activity, enzyme covalently coupled to the CNBr-activated Sepharose shows about 30% of maximal activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A shows about 35% of maximal activity, pH 11: native enzyme shows about 25% of maximal activity, enzyme covalently coupled to the CNBr-activated Sepharose shows about 30% of maximal activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A shows about 60% of maximal activity Ananas comosus