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Literature summary for 3.4.22.32 extracted from

  • Harrach, T.; Eckert, K.; Schulze-Forster, K.; Nuck, R.; Grunow, D.; Maurer, H.R.
    Isolation and partial characterization of basic proteinases from stem bromelain (1995), J. Protein Chem., 14, 41-52.
    View publication on PubMed
Search for more literature for 3.4.22.32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F9 Ananas comosus
2.3
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F4 Ananas comosus
2.42
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F5 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23430
-
 Ananas comosus, enzyme form F9, mass spectroscopy Ananas comosus
24400
-
 Ananas comosus, enzyme form F4, mass spectroscopy Ananas comosus
24470
-
 Ananas comosus, enzyme form F5, mass spectroscopy Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
 at least 8 basic proteolytically active components
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme form F4 and F5 contain fucose, N-acetylglucosamine, xylose and mannose in the ratio of 1.0:2.0:1.0:2.0, but only 50% of the proteins seem to be glycosylated, enzyme form F9 is unglycosylated Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F5 Ananas comosus
3.94
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F9 Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 L-pyroglutamyl-L-Phe-L-Leu 4 nitroanilide, enzyme form F4 and F5 Ananas comosus
7
-
 about, L-pyroglutamyl-L-Phe-L-Leu 4-nitroanilide, enzyme form F9 Ananas comosus