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Literature summary for 3.4.22.32 extracted from
- Fractionation, purification, and some properties of proteolytic enzymes from stem bromelain (1971), Agric. Biol. Chem., 35, 1419-1430.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-dimercapto-1-propanol | not as effective in activation as cysteine | Ananas comosus |  |
| cysteine | inactive unless treated with | Ananas comosus | |
| DTT | not as effective in activation as cysteine | Ananas comosus | |
| Thioglycol | not as effective in activation as cysteine | Ananas comosus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Ananas comosus | |
| iodoacetic acid | - |
Ananas comosus | |
| PCMB | - |
Ananas comosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 57 | - |
benzoyl-L-Arg ethyl ester | acidic enzyme form | Ananas comosus | |
| 67 | - |
benzoyl-L-Arg amide | acidic enzyme form | Ananas comosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
2 enzyme forms: pI 9.45 (basic form), pI 4.7 (acidic form) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 enzyme forms with pI of 9.45 (basic form) and pI 4.7 (acidic form) | Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-L-Arg amide + H2O | - |
Ananas comosus | Benzoyl-L-Arg + NH3 | - |
? | |
| Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | Benzoyl-L-Arg + ethanol | - |
? | |
| casein + H2O | - |
Ananas comosus | hydrolyzed casein | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 7, 30 min, stable up to | Ananas comosus |
| 50 | - |
pH 5, 60 min, stable | Ananas comosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | casein | Ananas comosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 5: about 40% of activity maximum, 10.0: about 70% of activity maximum | Ananas comosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 4°C, 24 h, stable | Ananas comosus |
| 5 | - |
50°C, 60 min, stable | Ananas comosus |
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