Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.32 extracted from

  • Ota, S.; Horie, K.; Hagino, F.; Hashimoto, C.; Date, H.
    Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant (1972), J. Biochem., 71, 817-830.
    View publication on PubMed
Search for more literature for 3.4.22.32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
 benzoyl-L-Arg amide enzyme form I-1 Ananas comosus
6
-
 benzoyl-L-Arg amide enzyme form II-2 Ananas comosus
6.6
-
 benzoyl-L-Arg amide enzyme form II-1 Ananas comosus
9.5
-
 benzoyl-L-Arg amide enzyme form I-2 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 Ananas comosus, enzyme form I-2, II-1 and II-2, gel filtration Ananas comosus
28000
-
 Ananas comosus, enzyme form I-1, gel filtration Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
 enzyme forms: I-1, I-2, II-1, II-2
-

Purification (Commentary)

Purification (Comment) Organism
enzyme forms: I-1, I-2, II-1, II-2 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-L-Arg amide + H2O
-
 Ananas comosus Benzoyl-L-Arg + NH3
-
 ?
casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?
L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0039
-
 benzoyl-L-Arg amide enzyme form I-2 Ananas comosus
0.0046
-
 benzoyl-L-Arg amide enzyme form I-1 Ananas comosus
0.018
-
 benzoyl-L-Arg amide enzyme form II-2 Ananas comosus
0.027
-
 benzoyl-L-Arg amide enzyme form II-1 Ananas comosus
0.87
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F4 Ananas comosus