Any feedback?
Literature summary for 3.4.22.29 extracted from
- Regulation of enterovirus 2A protease-associated viral IRES activities by the cell's ERK signaling cascade implicating ERK as an efficiently antiviral target (2017), Antiviral Res., 143, 13-21 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vitro transcription of transcript RNAs from the mono-cistronic reporter plasmid, the bi-cistronic reporter plasmid, the wild type EV-A71 replicon, the EV-A71 AITTL deleted replicon, the EV-A71 2Apro mutated replicon, and the EV-A71 2Apro deleted replicon | Enterovirus A71 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H21N/D39E/C110A | site-directed mutagenesis, a EV-A71 2Apro deleted replicon plasmid is constructed by deletion mutagenesis of the wild-type EV-A71 replicon by mutation at three positions (mutations of H21N, D39E, and C110A) of the 2Apro coding sequence | Enterovirus A71 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellular eukaryotic translation initiation factor 4G + H2O | Enterovirus A71 | - |
? | - |
? |  |
| EV-A71 viral polyprotein + H2O | Enterovirus A71 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterovirus A71 | - |
EV-A71 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellular eukaryotic translation initiation factor 4G + H2O | - |
Enterovirus A71 | ? | - |
? | |
| cellular eukaryotic translation initiation factor 4G + H2O | eIF4GI, trans-cleavage | Enterovirus A71 | ? | - |
? | |
| EV-A71 viral polyprotein + H2O | - |
Enterovirus A71 | ? | - |
? | |
| EV-A71 viral polyprotein + H2O | cis-cleavage | Enterovirus A71 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2A protease | - |
Enterovirus A71 |
| 2Apro | - |
Enterovirus A71 |
| EV-A71 2Apro | - |
Enterovirus A71 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | viral 2Apro proteolytic activity is key process by disrupting the ERK signaling cascade. Disruption of the ERK signaling cascade interrupts viral 2Aprocatalysed processing of eIF4GI, overview. Inhibition of the ERK signaling cascade decreases 2Apro-mediated vIRES-dependent translation in other enteroviruses | Enterovirus A71 |
| metabolism | regulation of enterovirus 2A protease-associated viral IRES activities by the cell's ERK signaling cascade. The positive regulation of virus replication by the ERK cascade is mediated through effects on both the cis-cleavage of the viral polyprotein by 2Apro and its trans-cleavage of cellular eIF4GI. The ERK cascade positively regulates EV-A71-mediated cleavage of eIF4GI that establishes the cellular conditions which favour vIRES-dependent translation. This ERK-2Apro linked network coordinating vIRES efficiency is also found in other important human enteroviruses | Enterovirus A71 |
| physiological function | the enzymatic activity of virally encoded 2Apro is essential to the stimulation of vIRES mediated translation. Key role of ERK cascade in maintaining 2Apro proteolytic activity required to maximize EV IRES activity at different stages of viral lifecycle | Enterovirus A71 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
