Literature summary for 3.4.22.29 extracted from

Mu, Z.; Wang, B.; Zhang, X.; Gao, X.; Qin, B.; Zhao, Z.; Cui, S.
Crystal structure of 2A proteinase from hand, foot and mouth disease virus (2013), J. Mol. Biol., 425, 4530-4543.

Search for more literature for 3.4.22.29

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta cells	Enterovirus A71

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme C110A, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.1), 200 mM MgCl2 and 1% (w/v) polyethylene glycol 4000, at 22°C	Enterovirus A71

Protein Variants

Protein Variants	Comment	Organism
C110A	inactive	Enterovirus A71
D144A	the mutant shows increased activity compared to the wild type enzyme	Enterovirus A71
D144A/E145A	inactive	Enterovirus A71
DELTA140-143	inactive	Enterovirus A71
E145A	the mutant shows strongly reduced activity compared to the wild type enzyme	Enterovirus A71
R55A	the mutant shows reduced activity compared to the wild type enzyme	Enterovirus A71

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 20000, SDS-PAGE	Enterovirus A71

Organism

Organism	UniProt	Comment	Textmining
Enterovirus A71	Q66479	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography, Hitrap Q column chromatography, and Superdex 75 gel filtration	Enterovirus A71

Source Tissue

Source Tissue	Comment	Organism	Textmining

Subunits

Subunits	Comment	Organism
?	x * 20000, SDS-PAGE	Enterovirus A71

Synonyms

Synonyms	Comment	Organism
2A protease	-	Enterovirus A71
2Apro	-	Enterovirus A71

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Release 2023.1 (January 2023)