Cloned (Comment) | Organism |
---|---|
wild-type and mutant picornaviruses are isolated from HeLa cells transfected with in vitro-synthesized RNA using DEAE-dextran, RNA transcripts are produced from a picornavirus type 1 Mahoney P1M infectious clone, pT7M, or a picornavirus type 2 Lansing (P2L) infectious clone, pT7L | picornaviridae |
Protein Variants | Comment | Organism |
---|---|---|
V54A | a BseRI fragment 4742-6148 is transferred from Se1-3C-02 DNA to pT7M, leads to defects in trans but not cis cleavage | picornaviridae |
Y88L | the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells | picornaviridae |
Y88S | the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells | picornaviridae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | picornaviridae | 2Apro processes the viral polyprotein, and it cleaves a variety of host proteins, including the translation proteins eIF4GI, eIF4GII and poly(A)-binding protein | ? | - |
? | |
additional information | picornaviridae | important cellular substrate for 2Apro is eIF4G, cleavage of this translation protein leads to inhibition of host protein synthesis during picornavirus infection | ? | - |
? | |
additional information | picornaviridae | inhibitory role for 2Apro in the most downstream event in interferon signaling, the antiviral activities of interferon-stimulated genes | ? | - |
? | |
additional information | picornaviridae | proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
picornaviridae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | HeLa cells are infected | picornaviridae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 2Apro processes the viral polyprotein, and it cleaves a variety of host proteins, including the translation proteins eIF4GI, eIF4GII and poly(A)-binding protein | picornaviridae | ? | - |
? | |
additional information | important cellular substrate for 2Apro is eIF4G, cleavage of this translation protein leads to inhibition of host protein synthesis during picornavirus infection | picornaviridae | ? | - |
? | |
additional information | inhibitory role for 2Apro in the most downstream event in interferon signaling, the antiviral activities of interferon-stimulated genes | picornaviridae | ? | - |
? | |
additional information | proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells | picornaviridae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2Apro | - |
picornaviridae |
proteinase 2A | - |
picornaviridae |