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Literature summary for 3.4.22.29 extracted from

  • König, H.; Rosenwirth, B.
    Purification and partial characterization of poliovirus protease 2A by means of a functional assay (1988), J. Virol., 62, 1243-1250.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.29

General Stability

General Stability Organism
insoluble enzyme can be solubilized by 0.1% sarcosyl, solubilization enhances stability at 20°C, soluble enzyme is unstable in dilute solution, bovine serum albumin stabilizes Enterovirus C
stable to dialysis for more than 100 h at 4°C Enterovirus C

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ not (10 mM, esterase activity) Enterovirus C
Cd2+ inhibits esterase activity Enterovirus C
Cl- trace amounts eliminate activity Enterovirus C
Cu2+ weak, 1 mM, inhibits esterase activity Enterovirus C
iodoacetamide inhibits esterase activity Enterovirus C
leupeptin inhibits esterase activity Enterovirus C
Mg2+ not (10 mM, esterase activity) Enterovirus C
Mn2+ 10 mM, inhibits esterase activity; weak Enterovirus C
additional information no inhibition by pepstatin (esterase activity); no inhibition by PMSF (esterase activity) Enterovirus C
NEM inhibits esterase activity Enterovirus C
Pb2+
-
 Enterovirus C
tosyl-L-leucine-chloromethyl ketone inhibits esterase activity Enterovirus C
tosyl-L-phenylalanine-chloromethyl ketone inhibits esterase activity Enterovirus C
Zn2+ 1 mM, inhibits esterase activity Enterovirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His
-
 Enterovirus C

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Enterovirus C 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 x * 17000, SDS-PAGE Enterovirus C

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
 type 2 (P712-ch-2ab)
-

Purification (Commentary)

Purification (Comment) Organism
HeLa Ohio cells, 4-5 h after infection, to near homogeneity Enterovirus C
type 2 Enterovirus C

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell infected Enterovirus C
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
-
 Enterovirus C

Storage Stability

Storage Stability Organism
0°C, extremely unstable in crude extracts with a half-life of 2 h, 20% ethanol stabilizes, 4°C, 5 mg enzyme/ml, several months Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Leu-Gly-Gln-Met methyl ester + H2O esterase activity Enterovirus C Gly-Leu-Gly-Gln-Met + CH3OH
-
 ?
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His + H2O
-
 Enterovirus C Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala + Gly-Pro-Ser-Asp-Met-Tyr-Val-His
-
 ?

Subunits

Subunits Comment Organism
? x * 17000, SDS-PAGE Enterovirus C

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2
-
 t1/2 of esterase activity: more than 100 h Enterovirus C
20
-
 solubilization enhances stability at 20°C Enterovirus C
25
-
 t1/2 of esterase activity: 2 h Enterovirus C
37
-
 t1/2 of esterase activity: 5 min Enterovirus C

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.6 esterase activity Enterovirus C

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 below, irreversible inactivation, esterase activity Enterovirus C