Cloned (Comment) | Organism |
---|---|
expression of 3C mutants in Escherichia coli | picornaviridae |
Protein Variants | Comment | Organism |
---|---|---|
D32A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
D50A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
D58A/E63A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
D5A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
D64A | infectious virus can not be recovered from three of the mutated RNA in transfected HeLa cells | picornaviridae |
D85A | infectious virus can not be recovered from three of the mutated RNA in transfected HeLa cells | picornaviridae |
E121A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
E24A | the specific infectivity is reduced by 100fold relative to wild type RNA in transfected HeLa cells | picornaviridae |
E45A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
E53A/E55A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
E71A | infectious virus can not be recovered from three of the mutated RNA in transfected HeLa cells | picornaviridae |
E81A | the specific infectivity is equivalent to wild type RNA in transfected HeLa cells | picornaviridae |
E96A/D99A | the specific infectivity is reduced by 100fold relative to wild type RNA in transfected HeLa cells | picornaviridae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | picornaviridae | 3C protein and 3C-containing precursor proteins play roles in the virus lifecycle before, during and after RNA synthesis, enzyme has uridylylation activity on Vpg peptide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
picornaviridae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble mutant proteins are purified to greater than 80% purity in a single step by using a nickel nitrilotriacetic acid agarose spin column | picornaviridae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | - |
picornaviridae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
uridylylation efficiency for soluble 3C mutants is determined. 3C mutants D5A, E45A, E71A and E96A/D99A exhibit at least a 2fold reduction in Vpg peptide uridylylation. Mutants E24A and D50A exhibit an increase in Vpg peptide uridylylation on the order of 2fold greater than wild type 3C. Other mutants do not exhibit a substantial difference (2fold or greater) in Vpg peptide uridylylation activity relative to wild type | picornaviridae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 3C protein and 3C-containing precursor proteins play roles in the virus lifecycle before, during and after RNA synthesis, enzyme has uridylylation activity on Vpg peptide | picornaviridae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 3C dimer interacts with 3Dpol | picornaviridae |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
picornaviridae |