Literature summary for 3.4.22.28 extracted from

Kuyumcu-Martinez, N.M.; Van Eden, M.E.; Younan, P.; Lloyd, R.E.
Cleavage of poly(A)-binding protein by poliovirus 3C protease inhibits host cell translation: a novel mechanism for host translation shutoff (2004), Mol. Cell. Biol., 24, 1779-1790.

Search for more literature for 3.4.22.28

Application

Application	Comment	Organism
medicine	enteroviruses use a dual strategy for host translation shutoff, requiring cleavage of poly(A)-binding protein by 3Cpro and of translation iniation factor 4GI by 2Apro, inhibition of endogenous mRNA translation by 3Cpro in HeLa cells is independent of translation iniation factor 4GI or translation iniation factor 4GI cleavage	Enterovirus C

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HeLa cells	Enterovirus C
expression in BLR pLys DE3	Enterovirus C

Protein Variants

Protein Variants	Comment	Organism
Q537E	cleavage-resistant mutant of poly(A)-binding protein	Enterovirus C

Organism

Organism	UniProt	Comment	Textmining
Enterovirus C	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Enterovirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Poly(A)-binding protein + H2O	3Cpro removes the C-terminal domain that interacts with several translation factors, in HeLa cells 3Cpro inhibits translation of endogenous mRNAs and reporter RNA, 3Cpro-mediated translation inhibition can be restored by the addition of exogenous poly(A)-binding protein	Enterovirus C	?	-	r

Synonyms

Synonyms	Comment	Organism
3C protease	-	Enterovirus C
3Cpro	-	Enterovirus C

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Release 2023.1 (January 2023)