Application | Comment | Organism |
---|---|---|
drug development | 3Cpro as the molecular target to develop anti enterovirus 71 agents | enterovirus |
Cloned (Comment) | Organism |
---|---|
into pET-23a and expressed in Escherichia coli BL21 (DE3 pLysS) | enterovirus |
Protein Variants | Comment | Organism |
---|---|---|
C147S | no proteolytic activity in vivo and in vitro | enterovirus |
D85N | possesses proteolytic activity equal to that of wild type | enterovirus |
G155A | markedly reduced RNA binding activity | enterovirus |
H40D | no proteolytic activity in vivo and in vitro | enterovirus |
I86A | no proteolytic activity | enterovirus |
K156Q | markedly reduced RNA binding activity | enterovirus |
K82Q | possesses proteolytic activity equal to that of wild type | enterovirus |
R84K | possesses proteolytic activity equal to that of wild type | enterovirus |
R84Q | possesses proteolytic activity equal to that of wild type | enterovirus |
V154S | markedly reduced RNA binding activity, possesses proteolytic activity equal to that of wild type | enterovirus |
V154T | still binds to viral RNA though with lower efficiency than the wild type, possesses proteolytic activity equal to that of wild type | enterovirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
enterovirus | - |
enterovirus 71, TW2231/98 | - |
Purification (Comment) | Organism |
---|---|
- |
enterovirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 3Cpro possess RNA-binding activity, the regions responsible for RNA binding are KFRDI (positions 82-86) and VGK (positions 154-156) | enterovirus | ? | - |
? | |
polypeptide translated from plasmid DNA T7-delta3A-3B-3Cmut-delta3D | - |
enterovirus | delta3A-3B-3Cmut + 3Cmut-delta3D + 3B-3Cmut-delta3D | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
enterovirus |
3Cpro | - |
enterovirus |