Cloned (Comment) | Organism |
---|---|
cloned into a plasmid under the control of the bacteriophage T7 promoter, expressed both in an in vito transcription-translation system and in vivo in an Escherichia coli system containig an inducible T7 RNA polymerase | Foot-and-mouth disease virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | recombinant enzyme | Foot-and-mouth disease virus | 16020 | - |
soluble | recombinant enzyme | Foot-and-mouth disease virus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Foot-and-mouth disease virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
foot-and-mouth-disease virus P1 precursor polyprotein + H2O | recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1 processing | Foot-and-mouth disease virus | mature structural foot-and-mouth-disease virus proteins | VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme) | ? | |
foot-and-mouth-disease virus P1-2A polyprotein + H2O | recombinant foot-and-mouth-disease virus enzyme, cleaves VP3-VP1 site initially, followed by VP0-VP3 and VP1-2A site (least efficiently), kinetic of P1-processing | Foot-and-mouth disease virus | mature structural foot-and-mouth-disease virus proteins | VPO-VP3 + VP1-2A (enzyme from supernatant), VPO + VP3 + VP1 (pellet enzyme) | ? | |
foot-and-mouth-disease virus P2 precursor protein + H2O | - |
Foot-and-mouth disease virus | foot-and-mouth-disease virus protein 2B + foot-and-mouth-disease virus protein 2C + foot-and-mouth-disease virus protein 2A-2B | - |
? |