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Literature summary for 3.4.22.28 extracted from

  • Hämmerle, T.; Hellen, C.U.T.; Wimmer, E.
    Site-directed mutagenesis of the putative catalytic triad of poliovirus 3C proteinase (1991), J. Biol. Chem., 266, 5412-5416.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli or BL21(D3) Enterovirus C

Protein Variants

Protein Variants Comment Organism
C147S inactive mutant enzyme Enterovirus C
E71Q inactive mutant enzyme Enterovirus C
H40Y inactive mutant enzyme Enterovirus C

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
recombinant enzyme
-
Enterovirus C
-
type 2, i.e. Sabin strain
-
Enterovirus C
-
wild-type and catalytic site-directed mutant D85N
-
Enterovirus C Sabin
-
type 2, i.e. Sabin strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O corresponding to amino acid sequence flanking 2C/3A cleavage site, wild-type and mutant D85N enzyme Enterovirus C acetyl-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2
-
?
acetyl-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O corresponding to amino acid sequence flanking 2C/3A cleavage site, wild-type and mutant D85N enzyme Enterovirus C Sabin acetyl-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2
-
?
poliovirus 3ABC precursor polyprotein + H2O in-cis cleavage, autocatalytical processing, cleavage sites: 3A/3B and 3B/3C, in-cis cleavage ability of D85N mutant severely impaired Enterovirus C poliovirus protein 3A + poliovirus protein 3B + poliovirus protein 3C poliovirus protein 3B is only 22 amino acids long and not detected in the assay ?
poliovirus 3ABC precursor polyprotein + H2O in-cis cleavage, autocatalytical processing, cleavage sites: 3A/3B and 3B/3C, in-cis cleavage ability of D85N mutant severely impaired Enterovirus C Sabin poliovirus protein 3A + poliovirus protein 3B + poliovirus protein 3C poliovirus protein 3B is only 22 amino acids long and not detected in the assay ?
poliovirus polyprotein fragment corresponding to 2BC coding region + H2O in-trans cleavage, wild-type and mutant D85N enzyme Enterovirus C ?
-
?
poliovirus polyprotein fragment corresponding to 2BC coding region + H2O in-trans cleavage, wild-type and mutant D85N enzyme Enterovirus C Sabin ?
-
?