Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli or BL21(D3) | Enterovirus C |
Protein Variants | Comment | Organism |
---|---|---|
C147S | inactive mutant enzyme | Enterovirus C |
E71Q | inactive mutant enzyme | Enterovirus C |
H40Y | inactive mutant enzyme | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
recombinant enzyme | - |
Enterovirus C | - |
type 2, i.e. Sabin strain | - |
Enterovirus C | - |
wild-type and catalytic site-directed mutant D85N | - |
Enterovirus C Sabin | - |
type 2, i.e. Sabin strain | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O | corresponding to amino acid sequence flanking 2C/3A cleavage site, wild-type and mutant D85N enzyme | Enterovirus C | acetyl-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 | - |
? | |
acetyl-Met-Glu-Ala-Leu-Phe-Gln-Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 + H2O | corresponding to amino acid sequence flanking 2C/3A cleavage site, wild-type and mutant D85N enzyme | Enterovirus C Sabin | acetyl-Met-Glu-Ala-Leu-Phe-Gln + Gly-Pro-Leu-Gln-Tyr-Lys-Asp-Leu-NH2 | - |
? | |
poliovirus 3ABC precursor polyprotein + H2O | in-cis cleavage, autocatalytical processing, cleavage sites: 3A/3B and 3B/3C, in-cis cleavage ability of D85N mutant severely impaired | Enterovirus C | poliovirus protein 3A + poliovirus protein 3B + poliovirus protein 3C | poliovirus protein 3B is only 22 amino acids long and not detected in the assay | ? | |
poliovirus 3ABC precursor polyprotein + H2O | in-cis cleavage, autocatalytical processing, cleavage sites: 3A/3B and 3B/3C, in-cis cleavage ability of D85N mutant severely impaired | Enterovirus C Sabin | poliovirus protein 3A + poliovirus protein 3B + poliovirus protein 3C | poliovirus protein 3B is only 22 amino acids long and not detected in the assay | ? | |
poliovirus polyprotein fragment corresponding to 2BC coding region + H2O | in-trans cleavage, wild-type and mutant D85N enzyme | Enterovirus C | ? | - |
? | |
poliovirus polyprotein fragment corresponding to 2BC coding region + H2O | in-trans cleavage, wild-type and mutant D85N enzyme | Enterovirus C Sabin | ? | - |
? |