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Literature summary for 3.4.22.27 extracted from

  • Kirschke, H.; Wiederanders, B.; Brömme, D.; Rinne, A.
    Cathepsin S from bovine spleen. Purification, distribution, intracellular localization and action on proteins (1989), Biochem. J., 264, 467-473.
    View publication on PubMed
Search for more literature for 3.4.22.27

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bos taurus

General Stability

General Stability Organism
the enzyme is partially destroyed by autolysis of the homogenate at pH 4.2 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic vesicle
-
 Bos taurus 31410
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 gel filtration Bos taurus
23700
-
 x * 23700, calculation from nucleotide sequence Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is expected to contain carbohydrate Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney concentrated to the cells of the proximal tubule Bos taurus
-
lung
-
 Bos taurus
-
lymph node
-
 Bos taurus
-
spleen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.582
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-10°C, 0.012-0.04 mM cathepsin S, 100 mM sodium acetate buffer, pH 5.5, 1 mM EDTA, stable for 8 months. In presence of 0.5 mM Hg2+ and 1 mM EDTA rapid decrease of about 50% of the activity which then remains unchanged for 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O
-
 Bos taurus ?
-
 ?
azocasein + H2O high activity in presence of urea at pH 5.5 Bos taurus ?
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Bos taurus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Collagen + H2O
-
 Bos taurus ?
-
 ?
haemoglobin + H2O
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23700, calculation from nucleotide sequence Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 5.5-7.0, in presence for dithiothreitol and EDTA stable for 240 min. 10-12% of the enzyme activity is lost after 60 min at pH 7.5 or pH 4.0. In absence of dithiothreitol and EDTA the enzyme is inactivated at pH 4.0 by 70% after 60 min Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA, 70% inactivation after 60 min in absence of dithiothreitol and EDTA Bos taurus
5.5 7 40°C, stable for 240 min in presence of dithiothreitol and EDTA Bos taurus
7.5
-
 40°C, 10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus isoelectric focusing
-
 7