Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 76% inhibition at about 40 mM | Homo sapiens | |
benzamidine | strong inhibition | Homo sapiens | |
CaCl2 | 91% inhibition at about 40 mM | Homo sapiens | |
EDTA | low inhibition | Homo sapiens | |
HgCl2 | complete inhibition at about 40 mM | Homo sapiens | |
MgCl2 | 81% inhibition at about 40 mM | Homo sapiens | |
MgSO4 | 68% inhibition at about 40 mM | Homo sapiens | |
additional information | no or poor inhibition by iodoacetamide and E-64 | Homo sapiens | |
NaCl | 12-21% inhibition, respectively | Homo sapiens | |
pepstatin A | low inhibition | Homo sapiens | |
Phenylmethylsulfonylfluoride | strong inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01182 | - |
tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
0.2265 | - |
PQVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
0.2675 | - |
QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
0.3463 | - |
QSPVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
0.3933 | - |
LPAPR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inactive factor X + H2O | Homo sapiens | activation of blood coagulation factor X, degradation of factor X during 24 h | active factor Xa + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from human placental membranes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tumor cell | Homo sapiens | - |
placental membrane | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-acetyl-GYPGQV + H2O | - |
Homo sapiens | 7-methoxycoumarin-4-acetate + GYPGQV | - |
? | |
7-methoxycoumarin-4-acetyl-IVGGQE + H2O | - |
Homo sapiens | 7-methoxycoumarin-4-acetate + IVGGQE | - |
? | |
7-methoxycoumarin-4-acetyl-SFLLRN + H2O | - |
Homo sapiens | 7-methoxycoumarin-4-acetate + SFLLRN | - |
? | |
7-methoxycoumarin-4-acetyl-VSQKSP + H2O | - |
Homo sapiens | 7-methoxycoumarin-4-acetate + VSQKSP | - |
? | |
7-methoxycoumarin-4-acetyl-VYQPQP + H2O | - |
Homo sapiens | 7-methoxycoumarin-4-acetate + VYQPQP | - |
? | |
collagen type IV + H2O | - |
Homo sapiens | ? | - |
? | |
Fibronectin + H2O | enzyme CP cleaves fibronectin between Ala and Val residues, in regions rich in Pro and Gln residues. The 45 and 60 kDa fragments have the same N-terminal sequence, generated by cleavage of the bond between Ala292 and Val293 | Homo sapiens | ? | - |
? | |
inactive factor X + H2O | activation of blood coagulation factor X, degradation of factor X during 24 h | Homo sapiens | active factor Xa + ? | - |
? | |
inactive factor X + H2O | activation of blood coagulation factor X (57.3 kDa), cleavage of factor X at the conventional activation site, i.e. between Arg52 and Ile53, in the heavy chain | Homo sapiens | active factor Xa + ? | - |
? | |
Laminin + H2O | - |
Homo sapiens | ? | - |
? | |
LPAPR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | LPAPR + 7-amino-4-methylcoumarin | - |
? | |
additional information | substrate specificity analysis, mass spectrometric detection of cleavage products. No activity with tert-benzyloxycarbonyl-QSPVR-7-amido-4-methylcoumarin and TLDPR-7-amido-4-methylcoumarin. Enzyme CP shows very little cysteine protease activity, it appears to exhibit mixed protease activity, but acts predominantly as a serine protease | Homo sapiens | ? | - |
? | |
PQVR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | PQVR + 7-amino-4-methylcoumarin | - |
? | |
QSPVR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | QSPVR + 7-amino-4-methylcoumarin | - |
? | |
QVR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | QVR + 7-amino-4-methylcoumarin | - |
? | |
tert-butoxycarbonyl-QVR-7-amido-4-methylcoumarin + H2O | best synthetic substrate | Homo sapiens | tert-butoxycarbonyl-QVR + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.989 | - |
tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
1.147 | - |
QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
2.496 | - |
QSPVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
3.942 | - |
LPAPR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
4.578 | - |
PQVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | cancer procoagulant (CP) is a protease present in malignant tumours, but not normal tissue, is a known activator of coagulation factor X (FX). degradation of FX by CP occurs at a much slower rate than degradation by conventional activators. Cancer procoagulant has a function in cancer progression. The enzyme is not essential for either cancer-associated blood coagulation or the degradation of extracellular matrix proteins. The protease may be required for the proteolytic activation of membrane receptors | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.288 | - |
QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
7.208 | - |
QSPVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
10.02 | - |
LPAPR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
20.21 | - |
PQVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens | |
83.67 | - |
tert-benzyloxycarbonyl-QVR-7-amido-4-methylcoumarin | pH 7.4, 37°C | Homo sapiens |