Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme depends on a thiolate ion for activity | Carica papaya |
Protein Variants | Comment | Organism |
---|---|---|
G27E | site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position | Carica papaya |
G73R | site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position, decreased affinity for cystatin C | Carica papaya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Alpha-macroglobulin | - |
Carica papaya | |
E64 | inactivation | Carica papaya | |
iodoacetamide | slow inactivation | Carica papaya | |
iodoacetate | slow inactivation | Carica papaya | |
additional information | no inhibition by most cystatins | Carica papaya | |
papaya cystatin | - |
Carica papaya | |
Peptidyl diazomethanes | dependent on the substrate | Carica papaya | |
Pro-peptide | the enzyme is inhibited by its own pro region after cleavage, also pro-peptide of other C1 peptidase family enzymes can be inhibitory | Carica papaya |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | latex | Carica papaya | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23313 | - |
1 * 23313 | Carica papaya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Carica papaya | the enzyme is useful for the plant defense system | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | mature enzyme | Carica papaya |
proteolytic modification | the enzyme is synthesized as preproenzyme | Carica papaya |
Purification (Comment) | Organism |
---|---|
different methods, overview | Carica papaya |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Carica papaya | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chicken cystatin + H2O | - |
Carica papaya | ? | - |
? | |
Gly-4-nitroanilide + H2O | - |
Carica papaya | glycine + 4-nitroaniline | - |
? | |
Human cystatin C + H2O | - |
Carica papaya | ? | - |
? | |
additional information | the enzyme is useful for the plant defense system | Carica papaya | ? | - |
? | |
additional information | substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide | Carica papaya | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 23313 | Carica papaya |
More | three-dimensional structure comparison to other latex peptidase | Carica papaya |
Synonyms | Comment | Organism |
---|---|---|
Chymopapain M | - |
Carica papaya |
Glycyl endopeptidase | - |
Carica papaya |
More | the enzyme belongs to the C1A peptidase family | Carica papaya |
Papaya peptidase B | - |
Carica papaya |
Papaya proteinase IV | - |
Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
about | Carica papaya |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000034 | - |
papapya cystatin | - |
Carica papaya | |
0.00086 | - |
Pro-peptide | - |
Carica papaya |