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Literature summary for 3.4.22.25 extracted from

  • Buttle, D.J.
    Glycyl endopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2, 1132-1134.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme depends on a thiolate ion for activity Carica papaya

Protein Variants

Protein Variants Comment Organism
G27E site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position Carica papaya
G73R site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position, decreased affinity for cystatin C Carica papaya

Inhibitors

Inhibitors Comment Organism Structure
Alpha-macroglobulin
-
Carica papaya
E64 inactivation Carica papaya
iodoacetamide slow inactivation Carica papaya
iodoacetate slow inactivation Carica papaya
additional information no inhibition by most cystatins Carica papaya
papaya cystatin
-
Carica papaya
Peptidyl diazomethanes dependent on the substrate Carica papaya
Pro-peptide the enzyme is inhibited by its own pro region after cleavage, also pro-peptide of other C1 peptidase family enzymes can be inhibitory Carica papaya

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular latex Carica papaya
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23313
-
1 * 23313 Carica papaya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Carica papaya the enzyme is useful for the plant defense system ?
-
?

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein mature enzyme Carica papaya
proteolytic modification the enzyme is synthesized as preproenzyme Carica papaya

Purification (Commentary)

Purification (Comment) Organism
different methods, overview Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chicken cystatin + H2O
-
Carica papaya ?
-
?
Gly-4-nitroanilide + H2O
-
Carica papaya glycine + 4-nitroaniline
-
?
Human cystatin C + H2O
-
Carica papaya ?
-
?
additional information the enzyme is useful for the plant defense system Carica papaya ?
-
?
additional information substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide Carica papaya ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 23313 Carica papaya
More three-dimensional structure comparison to other latex peptidase Carica papaya

Synonyms

Synonyms Comment Organism
Chymopapain M
-
Carica papaya
Glycyl endopeptidase
-
Carica papaya
More the enzyme belongs to the C1A peptidase family Carica papaya
Papaya peptidase B
-
Carica papaya
Papaya proteinase IV
-
Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
about Carica papaya

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000034
-
papapya cystatin
-
Carica papaya
0.00086
-
Pro-peptide
-
Carica papaya