Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activation | Rattus norvegicus | |
sulfhydryl compounds | activation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Rattus norvegicus | |
iodoacetamide | - |
Rattus norvegicus | |
iodoacetate | - |
Rattus norvegicus | |
leupeptin | - |
Rattus norvegicus | |
additional information | no inhibition by pepstatin A, soybean trypsin inhibitor | Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
PCMB | - |
Rattus norvegicus | |
PMSF | weak | Rattus norvegicus | |
tosyl-L-lysine chloromethyl ketone | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus norvegicus | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
gel filtration | Rattus norvegicus |
33500 | - |
rat, liver | Rattus norvegicus |
35000 | - |
1 * 35000, liver | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Tyrosine aminotransferase + H2O | Rattus norvegicus | involved in generation of isozymic forms of tyrosine transferase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
kidney | Rattus norvegicus |
liver | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HTC cell | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
small intestine | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, in 50 mM sodium phosphate buffer, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, at least 2 months | Rattus norvegicus |
4°C, at somewhat higher ionic strength than 50 mM sodium phosphate, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, inactivation within 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Acid-denatured hemoglobin + H2O | - |
Rattus norvegicus | ? | - |
? | |
azocasein + H2O | - |
Rattus norvegicus | ? | - |
? | |
additional information | cytosolic or mitochondrial forms of aspartate aminotransferase | Rattus norvegicus | ? | - |
? | |
additional information | Nalpha-benzoyl-(DL)-Arg-beta-naphthylamide | Rattus norvegicus | ? | - |
? | |
additional information | little or no activity at 0øC with glucose 6-phosphate dehydrogenase, native ribonuclease A, native bovine serum albumin, cytochrome c or oxidized insulin B-chain | Rattus norvegicus | ? | - |
? | |
Performic acid-oxidized ribonuclease A + H2O | - |
Rattus norvegicus | Hydrolyzed performic acid-oxidized ribonuclease A | - |
? | |
Tyrosine aminotransferase + H2O | involved in generation of isozymic forms of tyrosine transferase | Rattus norvegicus | ? | - |
? | |
Tyrosine aminotransferase form I + H2O | enzyme converses native larger subunit to smaller subunit | Rattus norvegicus | Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide | - |
? | |
Tyrosine aminotransferase form I + H2O | thereby generating multiple forms of tyrosine aminotransferase | Rattus norvegicus | Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Rattus norvegicus |
monomer | 1 * 35000, liver | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
15 min, pH 6.5, 50% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
0°C | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | active in this range, negligible below pH 5 and above pH 8 | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
15 min, 37°C, 50% loss of activity | Rattus norvegicus |
7 | - |
above, 0°C, 1.25% glycerol, rapid loss of activity | Rattus norvegicus |
7.8 | - |
20 min, 0°C, inactivation | Rattus norvegicus |