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Literature summary for 3.4.22.2 extracted from

  • Homaei, A.; Samari, F.
    Investigation of activity and stability of papain by adsorption on multi-wall carbon nanotubes (2017), Int. J. Biol. Macromol., 105, 1630-1635 .
    View publication on PubMed
Search for more literature for 3.4.22.2

Application

Application Comment Organism
industry immobilization on multi-walled carbon nanotubes is beneficial to the industrial applications because of its potential to be easily separated from the end product at the end of the reaction, reuse for multiple times and allow the development of multiple enzyme reaction system Carica papaya

General Stability

General Stability Organism
compared to free papain, the enzyme non-covalently immobilized on multi-walled carbon nanotubes exhibits significantly improved thermal, pH and recycling stability Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 casein the Km value of the papain immobilized on multi-walled carbon nanotubes experiences a slight increase, which suggests that the multi-walled carbon nanotubes do not significantly hinder the access of papain to substrate or release of product Carica papaya
0.0063
-
 casein soluble enzyme, pH 7.5, 23°C Carica papaya
0.0065
-
 casein enzyme immobilized on multi-walled carbon nanotubes, pH 7.5, 23°C Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya P00784
-
-

Storage Stability

Storage Stability Organism
23°C, pH 7.5, the enzyme immobilized on multi-walled carbon nanotubes loses about 20% of its activity after 40 days, the soluble enzyme loses about 40% of its activity after 20 days Carica papaya
4°C, pH 7.5, the enzyme immobilized on multi-walled carbon nanotubes is stable for 60 days, the soluble enzyme loses about 65% of its activity after 60 days Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Carica papaya ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 soluble enzyme Carica papaya
70
-
 enzyme immobilized on multi-walled carbon nanotubes Carica papaya

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 65% of maximal activity, 70°C: about 80% of maximal activity, soluble enzyme Carica papaya
30 80 30°C: about 40% of maximal activity, 80°C: about 80% of maximal activity, enzyme immobilized on multi-walled carbon nanotubes Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 at 80°C, the enzyme immobilized on multi-walled carbon nanotubes retains more than 70% of the initial activity after 1 h incubation. A nearly complete inactivation of free papain is observed Carica papaya
90
-
 the enzyme immobilized on multi-walled carbon nanotubes retains above 50% of the initial activity after 1 h incubation while the activity of the free enzyme is completely inactivated after 1 h incubation Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 casein enzyme immobilized on multi-walled carbon nanotubes, pH 7.5, 23°C Carica papaya
1.7
-
 casein soluble enzyme, pH 7.5, 23°C Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 soluble enzyme Carica papaya
8
-
 enzyme immobilized on multi-walled carbon nanotubes Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity, soluble enzyme Carica papaya
4 12 pH 4.0: about 55% of maximal activity, pH 12.0: about 45% of maximal activity, enzyme immobilized on multi-walled carbon nanotubes Carica papaya

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 after 60 min of incubation the immobilized enzyme retains about 98% of its residual activity Carica papaya
3
-
 after 60 min of incubation the immobilized enzyme retains about 53% of its residual activity Carica papaya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
230.8
-
 casein enzyme immobilized on multi-walled carbon nanotubes, pH 7.5, 23°C Carica papaya
269.8
-
 casein soluble enzyme, pH 7.5, 23°C Carica papaya