Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Alphacoronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | the reversible unfolding/refolding process of the PLpro in the presence of urea is investigated. The zinc-binding domain of the enzyme starts to unfold at urea concentration below 0.35 M and reaches a first plateau at 1-3.5 M. At this stage, the palm and thumb core domains remain well folded but totally inactive | Alphacoronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) | Alphacoronavirus | |
1.84 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) | Alphacoronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alphacoronavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin-7-amido-4-trifluoromethylcoumarin + H2O | - |
Alphacoronavirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
papain-like protease | - |
Alphacoronavirus |
PLpro | - |
Alphacoronavirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Alphacoronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) | Alphacoronavirus | |
0.96 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) | Alphacoronavirus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) | Alphacoronavirus | |
1.08 | - |
ubiquitin-7-amido-4-trifluoromethylcoumarin | pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) | Alphacoronavirus |