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Literature summary for 3.4.22.2 extracted from

  • Zhao, Y.; Gutshall, L.; Jiang, H.; Baker, A.; Beil, E.; Obmolova, G.; Carton, J.; Taudte, S.; Amegadzie, B.
    Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells (2009), Protein Expr. Purif., 67, 182-189.
    View publication on PubMed

Application

Application Comment Organism
synthesis Fab antibody fragment production and purification by papain digestion of an intact monoclonal antibody. After digestion, separation of the Fab fragment from the Fc fragment and residual intact antibody is achieved using protein A affinity chromatography. The Fab fragments are of high quality suitable to produce diffraction quality crystals suitable for X-ray crystallographic analysis Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
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