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Literature summary for 3.4.22.2 extracted from

  • Homaei, A.A.; Sajedi, R.H.; Sariri, R.; Seyfzadeh, S.; Stevanato, R.
    Cysteine enhances activity and stability of immobilized papain (2010), Amino Acids, 38, 937-942.
    View publication on PubMed
Search for more literature for 3.4.22.2

Application

Application Comment Organism
synthesis immobilization of papain on Sepharose 6B in the presence of different concentrations of cysteine affect the enzyme activity depending on cysteine concentration. The maximum specific activity is observed when papain was immobilized with 200 mM cysteine. The immobilization process results in significant enhancement of stability to temperature and extreme pH. After immobilization, the optimum temperature of papain activity increases by 20 degrees from 60 to 80°C and its optimum pH activity shifts from 6.5 to 8.0. Catalytic efficiency and specific activity of the immobilized enzyme do not significantly change after immobilization Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00057
-
 casein papain immobilized on sepharose 6B in presence of 200 mM cysteine, pH 7.5, 22°C Carica papaya
0.00062
-
 casein free papain, pH 7.5, 22°C Carica papaya
0.00079
-
 casein papain immobilized on sepharose 6B, pH 7.5, 22°C Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Carica papaya ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 free enzyme Carica papaya
80
-
 enzyme immobilized on sepharose 6B in presence or absence of 200 mM cysteine Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00000028
-
 casein papain immobilized on sepharose 6B, pH 7.5, 22°C Carica papaya
0.00000035
-
 casein papain immobilized on sepharose 6B in presence of 200 mM cysteine, pH 7.5, 22°C Carica papaya
0.00000045
-
 casein free papain, pH 7.5, 22°C Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 free enzyme Carica papaya
8
-
 enzyme immobilized on sepharose 6B in presence or absence of 200 mM cysteine Carica papaya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00035
-
 casein papain immobilized on sepharose 6B, pH 7.5, 22°C Carica papaya
0.00061
-
 casein papain immobilized on sepharose 6B in presence of 200 mM cysteine, pH 7.5, 22°C Carica papaya
0.00064
-
 casein free papain, pH 7.5, 22°C Carica papaya