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Literature summary for 3.4.22.2 extracted from

  • Diaz-Mochon, J.J.; Planonth, S.; Bradley, M.
    From 10,000 to 1: Selective synthesis and enzymatic evaluation of fluorescence resonance energy transfer peptides as specific substrates for chymopapain (2009), Anal. Biochem., 384, 101-105.
    View publication on PubMed
Search for more literature for 3.4.22.2

Application

Application Comment Organism
medicine enzyme is used for a number of biomedical applications Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
0.027
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
0.1015
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
0.1015
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
0.119
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
0.119
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
0.2
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
0.2
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya ?
-
 ?
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O FRET 2, fluorescence resonance energy transfer peptide 2 Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
 ?
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya ?
-
 ?
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 + H2O FRET 1, fluorescence resonance energy transfer peptide 1 Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N6-(5-carboxyfluorescein))-NH2
-
 ?

Synonyms

Synonyms Comment Organism
papain
-
 Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Carica papaya
37
-
 pH 6.2 or pH 7.4, 10 min Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
243
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
475
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
1503
-
 methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
1773
-
 methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.4 10 min, 37°C Carica papaya