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Literature summary for 3.4.22.2 extracted from

  • Szabelski, M.; Stachowiak, K.; Wiczk, W.
    Influence of Me2SO and incubation time on papain activity studied using fluorogenic substrates (2001), Acta Biochim. Pol., 48, 995-1002.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dimethyl sulfoxide the number of active sites of papain decreases with increasing concentration of dimethyl sulfoxide whereas the incubation time, in a buffer containing 3% dimethyl sulfoxide does not affect the number of active sites. A rapid decrease of the initial reaction rate, by up to 30%, is observed between 1 and 2% dimethyl sulfoxide Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
Carica papaya ?
-
?
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans + H2O
-
Carica papaya Dabcyl-Lys-Phe-Gly + Gly-Ala-Ala-Edans
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.833
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C Carica papaya